Interleukin-4 production from human basophils is critically dependent on the storage conditions employed prior to stimulation

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
E AumüllerH Haas

Abstract

Marked variations in the interleukin-4 (IL-4)-producing capacity of basophils can be observed when aliquots from the same cell preparation are kept under different conditions before stimulation. The aim of this study was to identify factors affecting the functional activity of basophils and to determine optimal storage conditions. Healthy blood donors. Aliquots of purified basophils were kept for different time intervals on ice or at 37 degrees C, in buffer or culture medium, respectively. Following subsequent stimulation with anti-IgE, IL-4 release was determined. Upon storage times up to 4 h, basophils produced more IL-4 when kept at 37 degrees C as compared to 4 degrees C. Surprisingly, buffer was superior compared to culture medium for storage. When the storage time was 20 h, IL-4 release was reduced significantly under all conditions studied. The storage conditions considerably affect basophil IL-4 release and thus should taken into account when comparing results.

Citations

Aug 19, 2010·Hybridoma·Ling YangJie Zhu
Aug 21, 2013·The Journal of Allergy and Clinical Immunology·Donald W MacGlashan
Dec 20, 2011·The Journal of Allergy and Clinical Immunology·Markus BöhmUlrike Raap
Aug 12, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sohee KimBooki Min

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