PMID: 7523534Oct 1, 1994Paper

Interleukin-5 messenger RNA and immunoreactive protein expression by activated eosinophils in lesional atopic dermatitis skin

The Journal of Investigative Dermatology
Y TanakaM Capron

Abstract

The main cellular sources of interleukin-5 (IL-5) are T lymphocytes and mast cells. Recently, IL-5 mRNA has been identified in eosinophils from patients with celiac disease, eosinophilic heart diseases, and asthma. In an attempt to determine whether IL-5 is generated by eosinophils in atopic dermatitis we have used i) in situ hybridization with 35S-labeled IL-5 RNA probe combined with immunohistochemistry using a monoclonal antibody (MoAb) (EG2) directed against the activated form of Eosinophil Cationic Protein (ECP) and ii) double-immunostaining with anti-IL-5 MoAb and polyclonal anti-ECP antibody. We found that dermal eosinophils from lesional atopic dermatitis skin express IL-5 mRNA and protein. Moreover, highly purified blood eosinophils were also labeled with anti-IL-5 antibodies. The expression of IL-5 by eosinophils in atopic dermatitis might suggest an autocrine pathway of eosinophil differentiation and activation.

Citations

Oct 31, 1996·Annals of the New York Academy of Sciences·B LamkhiouedM Capron
Oct 23, 1997·The American Journal of Dermatopathology·T OlivryP F Moore
Jun 15, 2006·International Archives of Allergy and Immunology·Atsuko ItakuraKiyoshi Takatsu
Dec 1, 2006·The Journal of International Medical Research·H AkamatsuK Matsunaga
Jun 3, 1999·The British Journal of Dermatology·M J RicoR P Hall
Sep 25, 2014·Dose-response : a Publication of International Hormesis Society·Noriko Shimura, Shuji Kojima
May 29, 1998·The British Journal of Dermatology·M CaproniP Fabbri
Nov 19, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·N A MolfinoG P Geba
Nov 8, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Min Ju Kim, Se-Young Choung
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