Interleukin-6 is an autocrine growth factor for folliculostellate-like TtT/GF mouse pituitary tumor cells

Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association
U RennerG K Stalla

Abstract

Within the pituitary, folliculostellate (FS) cells are considered to regulate the intercellular communication between endocrine cells by paracrine mechanisms. One of the possible paracrine factors involved, could be interleukin-6 (IL-6) which is produced by these cells. Since IL-6 has been shown to be a growth factor of pituitary cells, we have determined whether IL-6 can also influence FS cell proliferation. To test this, a FS cell-like mouse pituitary cell line (TtT/GF cells) was used that exhibits most characteristics of normal FS cells. Under serum-free conditions the proliferation of TtT/GF cells is critically dependent on the initial seeding density: cells seeded at low density do not grow at all whereas cells seeded at high density proliferate with maximal doubling times of 34 hrs. Mouse IL-6 (mIL-6) stimulated only low density cell cultures in a dose and time dependent manner. For cells seeded at high density, exogenously added IL-6 may have failed to stimulate growth because of endogenously produced mIL-6. Conditioned medium from TtT/GF cells, in which mIL-6 concentrations up to 1017 pg/ml were measured, stimulated the proliferation of TtT/GF cells, indicating an autocrine growth stimulatory mechanism. A neutralizing m...Continue Reading

Citations

Apr 23, 2003·Molecular and Cellular Endocrinology·Alberto Carbia NagashimaEduardo Arzt
Oct 24, 1998·Domestic Animal Endocrinology·U RennerG K Stalla
Feb 5, 2010·Journal of Cell Science·Claire V HarperMichael R H White
Dec 9, 2014·International Journal of Endocrinology·Federica BarbieriTullio Florio
Jan 1, 2016·Biochemical Genetics·Melanie SapochnikEduardo Arzt
Feb 24, 2006·Journal of Neuroimmunology·Eliane Corrêa-de-SantanaWilson Savino
Jan 12, 1999·Frontiers in Neuroendocrinology·E ArztG K Stalla
Apr 7, 2017·Journal of Molecular Endocrinology·Melanie SapochnikEduardo Arzt
Mar 6, 2007·Neuroendocrinology·Damiana GiacominiEduardo Arzt
Oct 21, 2004·Mass Spectrometry Reviews·Xianquan Zhan, Dominic M Desiderio
Jun 20, 2020·Frontiers in Neuroendocrinology·Pedro MarquesMárta Korbonits

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