Interleukin-6 production by U373 MG, a human astrocytoma cell line: different pathways involved in substance P and lipopolysaccharide activation

Journal of Neuroimmunology
C PalmaS Manzini

Abstract

Substance P (SP) and lipopolysaccharide (LPS) stimulated interleukin-6 (IL-6) gene expression, as well as IL-6 protein secretion in the human astrocytoma cell line U373 MG. Staurosporine, an inhibitor of protein kinase C (PKC), entirely blocked SP- but not LPS-induced IL-6 release. In addition, the down regulation of PKC inhibited the SP response and only marginally altered LPS activation. Differently from SP, LPS-induced IL-6 release was markedly reduced by W7, a calmodulin antagonist. Moreover, SP interacted in a synergistic manner with LPS. Thus, neural (SP) and bacterial (LPS) mediators stimulate U373 MG IL-6 release via distinct, though not antagonistic, activation pathways.

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Citations

Nov 3, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Guglielmina ChimientiGabriella Pepe
May 26, 2005·Journal of Neuroimmunology·Alexandra Berger, Christopher J Paige
Apr 27, 2001·Neuroscience Letters·D J SanchezA O Martinez
May 27, 2005·The Journal of Pharmacology and Experimental Therapeutics·Hon-Wai KoonCharalabos Pothoulakis
Aug 2, 2011·Journal of Neuroinflammation·Ingo SchulzHans-Ulrich Demuth
Apr 1, 1998·Journal of Neuroimmunology·C Palma, S Manzini
Mar 1, 2000·Journal of Neuroimmunology·N J Van Wagoner, E N Benveniste
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Jun 10, 2000·The Journal of Biological Chemistry·I CastagliuoloC Pothoulakis
Apr 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy RasleyJuan Anguita

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