Interleukin-6 receptor superantagonist Sant7 inhibits TGF-beta-induced proliferation of human lung fibroblasts.

Cell Proliferation
Luca GallelliR Savino

Abstract

Both interleukin-6 (IL-6) and transforming growth factor-beta (TGF-beta) are crucially involved in fibrotic events that characterize interstitial lung diseases (ILD). Therefore, the aim of this study was to investigate in primary cultures of normal and fibrotic human lung fibroblasts (HLF), exposed to either IL-6 or TGF-beta1, the effects on phosphorylation of mitogen-activated protein kinases (MAPK) and cell growth of IL-6 signalling inhibition, performed by the IL-6 receptor superantagonist Sant7. MAPK phosphorylation was detected by Western blotting, HLF viability and proliferation were evaluated using the trypan blue staining and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, respectively. Sant7, at a concentration of 1 microg/mL, was capable of significantly inhibiting HLF proliferation and MAPK phosphorylation induced by cell exposure to IL-6 (100 ng/mL) or TGF-beta1 (10 ng/mL), whose actions were more evident in fibrotic cells. These findings suggest that, in HLFs derived from patients with ILDs, the proliferative mechanisms activated by TGF-beta1 are at least in part mediated by an increased release of IL-6, leading to phosphorylation-dependent MAPK activation. Such preliminary findings may thus...Continue Reading

References

Nov 24, 1995·Science·Z XiaM E Greenberg
Nov 1, 1994·American Journal of Respiratory Cell and Molecular Biology·K M FriesR P Phipps
May 1, 1994·American Journal of Respiratory and Critical Care Medicine·B CrestaniM F Kahn
Jun 1, 1996·Clinical Immunology and Immunopathology·I ShaharS Ben Efraim
Jan 1, 1997·Annual Review of Immunology·T Taga, T Kishimoto
Mar 10, 2001·Nature·L Chang, M Karin
Apr 11, 2003·Reviews of Physiology, Biochemistry and Pharmacology·D KamimuraT Hirano
Apr 26, 2003·American Journal of Respiratory Cell and Molecular Biology·Yuben P MoodleyDarryl A Knight
May 30, 2003·The Biochemical Journal·Peter C HeinrichFred Schaper
Feb 18, 2004·International Journal of Radiation Oncology, Biology, Physics·Nicole Barthelemy-BrichantBetty Nusgens
Aug 24, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Eleonora CavarraGiuseppe Lungarella
Jun 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Pierfrancesco TassoneKenneth C Anderson
Nov 1, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Chiharu TabataMichiaki Mishima
Feb 3, 2006·The European Respiratory Journal·P J Barnes
Oct 27, 2006·Proceedings of the American Thoracic Society·Jack GauldieDavid Warburton
Nov 1, 2006·Nature Clinical Practice. Rheumatology·Norihiro Nishimoto, Tadamitsu Kishimoto
Mar 24, 2007·American Journal of Respiratory Cell and Molecular Biology·Xiangde LiuStephen I Rennard

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Citations

Jun 3, 2011·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Ana Paula Moreira, Cory M Hogaboam
Mar 9, 2013·American Journal of Respiratory Cell and Molecular Biology·Ragini VittalDavid S Wilkes
Jul 16, 2013·BioMed Research International·Luca GallelliGirolamo Pelaia
Oct 6, 2009·Free Radical Biology & Medicine·R-M Liu, K A Gaston Pravia
Oct 4, 2011·Scandinavian Journal of Rheumatology·M Mohr, A M Jacobi
Sep 3, 2014·International Journal of Surgery·Alessandro VatrellaVincenzo Di Crescenzo
Mar 25, 2014·Pulmonary Pharmacology & Therapeutics·Hatem AlkhouriMichael Schuliga
Apr 6, 2013·International Wound Journal·Raffaele SerraStefano de Franciscis
Mar 28, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Raffaele SerraStefano de Franciscis
Aug 7, 2019·Antioxidants·Roberto CannataroErika Cione
Apr 16, 2020·Journal of Clinical Medicine·Giulia CassoneMarco Sebastiani
Mar 30, 2021·Expert Review of Clinical Immunology·Andreina ManfrediMarco Sebastiani

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