Interleukin-6 stimulates cell proliferation of rat pituitary clonal cell lines in vitro

Journal of Endocrinological Investigation
T SawadaA Miyake

Abstract

We investigated the effect of recombinant human IL-6 (rhIL-6) on cell proliferation using the MtT/E rat pituitary tumor cell line, which was recently established by Inoue et al. This cell line expresses the homeodomain protein Pit-1/GHF 1 and does not produce any significant amount of pituitary hormones, but retains its tumorigenicity by back-transplantation into rats, resulting in production of prolactin. MtT/E cells were seeded into Falcon 24-well plates at a density of 2 x 10(4) cells/well in a cultured medium, containing 10% horse serum and 2.5% fetal bovine, with test drug. After four-days (12 days for the time-course study) incubations, the cells were counted using a hemocytometer. Incubation for 4 days with rhIL-6 caused concentration-dependent stimulation of MtT/E cell growth and [3H]-thymidine incorporation into MtT/E cells. Addition of 20 ng/ml rhIL-6 to the culture medium stimulated MtT/E cell growth in a time-dependent manner, withdrawal of rhIL-6 from the culture medium reduced MtT/E cell growth, and re-addition of rhIL-6 to the culture medium again stimulated MtT/E cell growth. Among the cytokines tested, granulocyte colony-stimulating factor (rh G-CSF) also showed a slight but significant mitogenic activity on th...Continue Reading

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Citations

Oct 21, 2004·Mass Spectrometry Reviews·Xianquan Zhan, Dominic M Desiderio
Mar 6, 2007·Neuroendocrinology·Damiana GiacominiEduardo Arzt
Jan 23, 1999·International Reviews of Immunology·R C Gaillard
Jul 8, 2008·Journal of Neuroendocrinology·S Devnath, K Inoue
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Jun 20, 2020·Frontiers in Neuroendocrinology·Pedro MarquesMárta Korbonits
Jan 12, 1999·Frontiers in Neuroendocrinology·E ArztG K Stalla

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