PMID: 8591014Dec 1, 1995Paper

Interleukin-8 gene expression in the human colon epithelial cell line, HT-29, exposed to Entamoeba histolytica

The Korean Journal of Parasitology
J M KimC Y Kim

Abstract

The protozoan parasite, Entamoeba histolytica, is one of major causative agents of intestinal disease all over the world. In acute experimental infection, the early host response to E. histolytica is characterized by an infiltration of neutrophils. However, the chemotactic signal for this response is not well known. Based on the finding that human epithelial cells produce the potent neutrophil chemoattractant and activator, interleukin-8 (IL-8), IL-8 gene expression was examined thoroughly in human colon epithelial cells exposed to E. histolytica trophozoites. Cellular RNAs were extracted from HT-29 or Caco-2 human colon epithelial cells exposed to E. histolytica trophozoites for 30 minutes, 1 and 3 hours. IL-8 mRNA transcripts were measured by reverse transcriptional polymerase chain reaction (RT-PCR) using synthetic standard RNA. The number of IL-8 mRNA molecules increased from 30 minutes to 3 hours of exposure period, reaching 3.1 x 10(7) molecules/micrograms of total RNA. Expression pattern of IL-8 mRNA transcripts was parallel to the amounts of IL-8 protein measured by enzyme-linked immunosorbent assay (ELISA). Lysates of E. histolytica also induced expression of mRNA for IL-8 in colon epithelial cells. These results sugge...Continue Reading

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Citations

May 30, 2001·Trends in Parasitology·S L Stanley
Jun 14, 2002·Microbiology and Immunology·Hirotsugu MoritaJun-ichi Kurisaki
Jun 17, 2009·Parasite Immunology·R E Sierra-PuenteJ Ventura-Juárez
Nov 4, 2009·The Korean Journal of Parasitology·Seung-Yull Cho

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