Interleukin enhancer-binding factor 3 and HOXC8 co-activate cadherin 11 transcription to promote breast cancer cells proliferation and migration

Oncotarget
Yang ZhangYong Li

Abstract

Cadherin 11 (CDH11) expression is detected only in invasive breast cancer cells and aggressive breast cancer specimens. However, little is known about the molecular mechanisms of CDH11 transcriptional regulation. Here, we report that interleukin enhancer binding factor 3 (ILF3) interacts with Homeobox C8 (HOXC8) to activate CDH11 transcription in breast cancer cells. Using co-immunoprecipitation and mass spectrometry analyses, ILF3 is shown to interact with HOXC8 in breast cancer cells. We demonstrate that ILF3 binds to the CDH11 promoter on nucleotides -2982 ~ -2978 and -2602 ~ 2598 and interacts with HOXC8 to co-activate CDH11 transcription. We further show that ILF3 promotes proliferation and migration, at least partially, by facilitating CDH11 expression in breast cancer cells. Moreover, immunohistochemistry (IHC) shows that expression of CDH11, ILF3 and HOXC8 are all upregulated in breast cancer specimens compared to normal breast tissues. Importantly, the expression levels of CDH11, ILF3 and HOXC8 are elevated in the advanced stages of breast cancer, and high expression of CDH11, ILF3 and HOXC8 is associated with poor distant metastasis-free survival (DMFS) for breast cancer patients.

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Citations

Jun 25, 2019·Wiley Interdisciplinary Reviews. RNA·Yong Sun LeeYeon-Su Lee
Jan 8, 2020·Journal of Cellular Biochemistry·Rebecca S DeVauxJason I Herschkowitz
Feb 21, 2019·Experimental and Therapeutic Medicine·Jintao LiuXinyi Lu
Oct 28, 2020·Journal of Biomolecular Structure & Dynamics·Y Sheena MaryB Narayana
Nov 22, 2019·Journal of Experimental & Clinical Cancer Research : CR·Yan LiuYong-Hong Shi
Apr 17, 2019·Journal of Experimental & Clinical Cancer Research : CR·Jia-Xing ZhangDan Xie
Jan 3, 2020·Japanese Journal of Clinical Oncology·Ming-Kun ChenCun-Dong Liu
Apr 27, 2021·Experimental Biology and Medicine·Mingfei XuShishan Deng
Apr 30, 2021·Technology in Cancer Research & Treatment·Ming WangGuoyong Yin
Jul 24, 2021·OncoTargets and Therapy·Bi-Tan ZhengBo-Hua Su

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Methods Mentioned

BETA
co-immunoprecipitation
immunoprecipitation
Co-IP
confocal microscopy
PCR
ChIP
transfection

Software Mentioned

GraphPad Prism
cBioPortal

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