PMID: 9184656Jun 1, 1997Paper

Interleukin (IL)-1 beta and IL-1 beta mRNA expression in normal and diseased skeletal muscle assessed by immunocytochemistry, immunoblotting and reverse transcriptase-nested polymerase chain reaction

Journal of Neuropathology and Experimental Neurology
L BélecR K Gherardi

Abstract

To confirm the production of IL-1 beta and to optimize detection and semiquantitation of IL-1 beta mRNA by polymerase chain reaction (PCR) techniques in skeletal muscle tissue, immunocytochemistry, immunoblotting and several procedures of RNA extraction and reverse transcription (RT)-PCR amplification were used on muscle samples from 12 patients with conditions associated with local production of IL-1 beta (AZT myopathy: 6 patients; sarcoid myopathy: 6 patients) and from 9 patients with normal muscle used as controls. Abundant IL-1 beta immunoreactivities, corresponding to both pro IL-1 beta and mature IL-1 beta as assessed by immunoblotting, were observed in all diseased muscles, either in inflammatory cells (sarcoid myopathy) or in atrophic muscle fibers (AZT myopathy). Acid guanidinium isothiocyanate phenol-chloroform extraction of RNA appeared less efficient for IL-1 beta mRNA detection by RT-PCR than proteinase K digestion followed by phenol-chloroform extraction. Even using the latter procedure, RT-single PCR for IL-1 beta mRNA was puzzlingly negative in all cases but one; in contrast, RT-nested PCR specified by DNA enzyme immunoassay yielded detection of IL-1 beta mRNA in all diseased muscles and in occasional controls, ...Continue Reading

Citations

Aug 19, 1999·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·D J RossS C Jordan
Jul 25, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Laurent BélecChristopher Payan
Jun 20, 2001·Acta Physiologica Scandinavica·M B Reid, Y P Li
Apr 29, 1998·Neuropathology and Applied Neurobiology·H LepidiJ F Pellissier
Jun 7, 2005·Acta Neuropathologica·Boel De PaepeJan L De Bleecker
Dec 23, 2006·Journal of Applied Physiology·Theodoros Vassilakopoulos, Sabah N A Hussain
Jan 22, 2005·Current Opinion in Critical Care·Theodoros VassilakopoulosSpyros Zakynthinos
Oct 31, 2002·American Journal of Respiratory and Critical Care Medicine·Theodoros VassilakopoulosCharis Roussos

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