Abstract
Interleukin (IL)-1beta-treated rat islets of Langerhans were exposed in vitro either to the imidazoline compound, Efaroxan, or to the selective inducible nitric oxide synthase (iNOS) inhibitor, 1400W, in a medium containing a high concentration of glucose (16.7 mmol/L). Our data have evidenced the following: (i) addition of Efaroxan to islet cultures inhibited IL-1beta activation of ICE (cysteine protease IL-1beta converting enzyme) while addition of 1400W did not; (ii) Efaroxan completely inhibited IL-1beta-induced suppression of insulin secretion and induction of iNOS mRNA transcripts, and, in addition, counteracted islet beta-cell protein profile alterations, Bax-cytochrome c translocation, caspase activation, and apoptosis; (iii) 1400W inhibited IL-1beta induction of iNOS, but failed to completely counteract the other cytotoxic effects; (iv) the two compounds, moreover, exerted different effects on manganese superoxide dismutase (MnSOD), in fact, while Efaroxan inhibited the early stimulatory effect of IL-1beta on MnSOD, 1400W did not. Thus, Efaroxan completely protected islet beta cells from damage caused by IL-1beta-induced toxicity, while compound 1400W only inhibited NO radical production without altering the cytokine's...Continue Reading
References
May 1, 1992·Endocrinology·L A BorgD L Eizirik
Dec 1, 1991·Endocrinology·N WelshS Sandler
Dec 10, 1990·FEBS Letters·C SouthernI C Green
Nov 1, 1989·Diabetologia·W SumoskiA Rabinovitch
Apr 1, 1986·Diabetes·G PapaccioS Frascatore
Jun 20, 1986·Science·K BendtzenM Svenson
Nov 11, 1988·Science·G H Wong, D V Goeddel
Dec 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A MasudaK Matsushima
May 1, 1994·The Journal of Clinical Investigation·D L EizirikC Hellerström
Nov 1, 1993·FEBS Letters·C A DelaneyI C Green
Nov 1, 1996·Diabetes·S V ZaitsevS Efendić
Feb 21, 1997·The Journal of Biological Chemistry·E P GarveyR G Knowles
Aug 1, 1997·Diabetes·G L WilsonS P LeDoux
Oct 23, 1997·The Journal of Experimental Medicine·G StassiM Trucco
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J M JürgensmeierJ C Reed
Jun 13, 1998·The Journal of Clinical Investigation·H E HohmeierC B Newgard
May 20, 1998·Diabetes·A C LowethN G Morgan
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·A R KhaledS K Durum
Feb 19, 2000·Journal of Cellular Biochemistry·G PapaccioM Galdieri
Feb 22, 2001·Biochemical and Biophysical Research Communications·A K AnderssonS Sandler
Mar 13, 2001·Biochemical and Biophysical Research Communications·A M EfanovS Efendic
Mar 29, 2001·Diabetes·S V ZaitsevP O Berggren
Jul 5, 2001·Journal of Autoimmunity·W L Suarez-PinzonA Rabinovitch
Dec 14, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·J R LakeyA Rabinovitch
Oct 9, 2002·European Journal of Endocrinology·Tobias Rydgren, Stellan Sandler
Dec 12, 2002·Diabetes·Suad EfendicSergei V Zaitsev
Apr 5, 2003·Journal of Neurochemistry·W J YoonB J Gwag
Sep 10, 2003·Endocrinology·Matilde TodaroGianpaolo Papaccio
Citations
Dec 8, 2006·Diabetologia·D MorganA R Carpinelli
Jul 2, 2010·Diabetologia·I RustenbeckM Tiedge
Jul 13, 2011·Rejuvenation Research·Ahmet CumaoğluÇimen Karasu
Dec 7, 2011·Journal of Ethnopharmacology·Teresa DiasAlexandra Paulo
May 28, 2009·Diabetes·Lars G GrunnetThomas Mandrup-Poulsen
Jun 12, 2018·British Journal of Pharmacology·Linlin ZhongWeijun Shen
Nov 16, 2018·PloS One·Kohei TakedaYuichi Izumi
Apr 1, 2009·Journal of Cellular Physiology·Alice SchwarznauLuis A Fernandez
Feb 22, 2007·Journal of Cellular Physiology·Marcella PedullàGianpaolo Papaccio
Feb 12, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kathrin MaedlerRaphael Roduit
Jul 14, 2018·Frontiers in Physiology·Kentaro MaruyamaEiji Nemoto
Jan 31, 2006·Nitric Oxide : Biology and Chemistry·Yoshihiko ChibaMiwa Misawa