Interleukin‑22 regulates the homeostasis of the intestinal epithelium during inflammation

International Journal of Molecular Medicine
Xinyan ZhangXueli Zhang

Abstract

Interleukin‑22 (IL‑22) has both pro‑inflammatory and anti‑inflammatory properties in a number tissues depending on the environment. Epithelial cells usually have a rapid turnover and are fueled by tissue stem cells. However, the question of whether IL‑22 regulates tissue homeostasis through the modulation of stem cells remains unanswered. In this study, we investigated the role of IL‑22 in the homeostasis of intestinal epithelial cells (IECs) during inflammation through a 3D organoid culture system. qPCR was performed to detect the changes in important gene transcriptions, and immunohistochemistry and western blot analysis were carried out to determine protein expression. As a result, we found that the expression of IL‑22 was synchronously altered with the damage of the intestine. IL‑22 treatment promoted cell proliferation and suppressed the cell differentiation of intestinal organoids. Surprisingly, IL‑22 also led to self‑renewal defects of intestinal stem cells (ISCs), thereby eventually resulting in the death of organoids. In examining the underlying mechanisms, we found that IL‑22 activated signal transducer and activator of transcription 3 (Stat3) phosphorylation and suppressed the Wnt and Notch signaling pathways. Import...Continue Reading

References

Sep 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L DumoutierJ C Renauld
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 23, 2004·Annual Review of Immunology·Sidney PestkaPaul B Fisher
Aug 17, 2004·Immunity·Kerstin WolkRobert Sabat
Sep 20, 2006·The Journal of Experimental Medicine·Spencer C LiangLynette A Fouser
Nov 8, 2006·Cell Research·Yeonseok ChungChen Dong
Jan 18, 2008·World Journal of Gastroenterology : WJG·Andreas Sturm, Axel U Dignass
Dec 23, 2008·Immunity·Lauren A ZenewiczRichard A Flavell
Jul 1, 2009·The Journal of Experimental Medicine·Geethanjali PickertChristoph Becker
Feb 26, 2010·Nature Reviews. Immunology·Lora V Hooper, Andrew J Macpherson
Nov 30, 2010·Cytokine & Growth Factor Reviews·Robert Sabat
Dec 21, 2010·Annual Review of Immunology·Wenjun OuyangSarah G Hymowitz
Mar 12, 2011·International Immunology·Lauren A Zenewicz, Richard A Flavell
Apr 27, 2011·The Journal of Experimental Medicine·Masahito KamanakaRichard A Flavell
Dec 19, 2012·Immunity·Kenya Honda
Apr 16, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lauren A ZenewiczRichard A Flavell
Jun 4, 2013·Nature Medicine·Johanna F DekkersJeffrey M Beekman
Jun 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Carlene L ZindlCasey T Weaver
Oct 23, 2014·Inflammatory Bowel Diseases·Daren LowEmiko Mizoguchi
Jan 7, 2015·World Journal of Gastroenterology : WJG·Lin-Jing LiBai-Sui Feng
Dec 10, 2015·Nature·Caroline A LindemansAlan M Hanash

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Methods Mentioned

BETA
IECs
X-ray
PCR
Protein Assay
IEC

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