Interleukins 4 and 13 and Their Receptors Are Differently Expressed in Gastrointestinal Tract Cancers, Depending on the Anatomical Site and Disease Advancement, and Improve Colon Cancer Cell Viability and Motility

Cancers
Iwona Bednarz-MisaMałgorzata Krzystek-Korpacka

Abstract

Immunosuppressive interleukins (IL)-4 and 13 may directly promote cancer but neither their status nor role in gastrointestinal tract is clarified. We aim at quantifying ILs and their receptors in paired normal-tumor samples (n = 49/51) and sera (n = 263), using immunoassays and RTqPCR, and screening for their effect on colonic cancer cells. Both ILs were elevated locally at protein level in all cancers but only IL13 transcripts in colon were upregulated. Interleukin and their receptor expression reflected cancer pathology to varying degrees, with the association frequently inverse and manifested in non-cancerous tissue. Positive correlation with cancer-promoting genes BCL2, BCLxL, HIF1A, VEGFA, ACTA2, CCL2, PTGS2, and CDKN1A, but not Ki67, was demonstrated, particularly for ILs' receptors. Circulating IL-4 was elevated in all, while IL-13 only in colorectal or esophageal cancers, reflecting their advancement. IL4Ra and IL13Ra1 transcripts were downregulated by hypoxia and, in Caco-2, also by IL-4. Interleukin stimulation slightly improved colonic cancer cell viability, weakly upregulating BCL2 and Ki67 in HCT116 and HT-29. It affected cell motility more markedly and was consistently accompanied by upregulation of claudin-2. Gas...Continue Reading

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Citations

Jul 8, 2020·International Journal of Molecular Sciences·Iwona Bednarz-MisaMałgorzata Krzystek-Korpacka
Nov 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mariusz G FleszarMałgorzata Krzystek-Korpacka
Apr 4, 2021·Cancers·Manuela Gonzalez-Aparicio, Carlos Alfaro

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Methods Mentioned

BETA
PCR
biopsies
xenograft
ELISA
flow cytometry
PCRs
electrophoresis

Software Mentioned

Genomed
BioPlex Manager
MedCalc
MRI Wound Healing Tool
ImageJ

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