Interlobular and intralobular mammary stroma: genotype may not reflect phenotype.

BMC Cell Biology
J M FlemingB K Vonderhaar

Abstract

The normal growth and function of mammary epithelial cells depend on interactions with the supportive stroma. Alterations in this communication can lead to the progression or expansion of malignant growth. The human mammary gland contains two distinctive types of fibroblasts within the stroma. The epithelial cells are surrounded by loosely connected intralobular fibroblasts, which are subsequently surrounded by the more compacted interlobular fibroblasts. The different proximity of these fibroblasts to the epithelial cells suggests distinctive functions for these two subtypes. In this report, we compared the gene expression profiles between the two stromal subtypes. Fresh normal breast tissue was collected from reduction mammoplasty patients and immediately placed into embedding medium and frozen on dry ice. Tissue sections were subjected to laser capture microscopy to isolate the interlobular from the intralobular fibroblasts. RNA was prepared and subjected to microarray analysis using the Affymetrix Human Genome U133 GeneChip. Data was analyzed using the Affy and Limma packages available from Bioconductor. Findings from the microarray analysis were validated by RT-PCR and immunohistochemistry. No statistically significant dif...Continue Reading

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Citations

Sep 9, 2010·Journal of Mammary Gland Biology and Neoplasia·Erin J McCaveBrian W Booth
Jun 26, 2010·Journal of Cellular Physiology·Jodie M FlemingBarbara K Vonderhaar
Jan 28, 2015·Endocrine-related Cancer·Denise K ReavesJodie M Fleming
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Oct 1, 2020·Breast Cancer Research : BCR·Mikkel MorsingLone Rønnov-Jessen
Oct 22, 2020·NPJ Breast Cancer·Natascia MarinoAnna Maria V Storniolo
Nov 5, 2020·Journal of Mammary Gland Biology and Neoplasia·Erin D Giles, Elizabeth A Wellberg
Nov 8, 2020·Seminars in Cell & Developmental Biology·Jakub SumbalZuzana Koledova

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Datasets Mentioned

BETA
GSE12306
AF201292

Methods Mentioned

BETA
PCR
laser capture microscopy
nucleotide-exchange
flow cytometry
electrophoresis

Software Mentioned

Bioconductor
Affy package
Affymetrix GeneChip Operating Software
NIH Image
Arcturus
Limma

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