Intermediate-conductance calcium-activated potassium channels in enteric neurones of the mouse: pharmacological, molecular and immunochemical evidence for their role in mediating the slow afterhyperpolarization

Journal of Neurochemistry
C B NeylonJohn B Furness

Abstract

Calcium-activated potassium channels are critically important in modulating neuronal cell excitability. One member of the family, the intermediate-conductance potassium (IK) channel, is not thought to play a role in neurones because of its predominant expression in non-excitable cells such as erythrocytes and lymphocytes, in smooth muscle tissues, and its lack of apparent expression in brain. In the present study, we demonstrate that IK channels are localized on specific neurones in the mouse enteric nervous system where they mediate the slow afterhyperpolarization following an action potential. IK channels were localized by immunohistochemistry on intrinsic primary afferent neurones, identified by their characteristic Dogiel type II morphology. The slow afterhyperpolarization recorded from these cells was abolished by the IK channel blocker clotrimazole. RT-PCR and western analysis of extracts from the colon revealed an IK channel transcript and protein identical to the IK channel expressed in other cell types. These results indicate that IK channels are expressed in neurones where they play an important role in modulating firing properties.

Citations

Jun 15, 2006·Journal of Molecular Histology·Michelle ThackerJohn B Furness
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jordan D T EngbersRay W Turner
Nov 21, 2008·Clinical and Experimental Pharmacology & Physiology·Shaun L SandowFrances Plane
Jan 15, 2014·Pflügers Archiv : European journal of physiology·Ray W Turner, Gerald W Zamponi
May 7, 2014·Pflügers Archiv : European journal of physiology·Ray W TurnerGerald W Zamponi
Feb 2, 2013·Digestive Diseases and Sciences·Edward G HawkinsHamid I Akbarali
Feb 13, 2009·Journal of Cellular and Molecular Medicine·Wolfgang A KunzeJohn Bienenstock
May 23, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K Nurgali
Nov 2, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J Ren, J J Galligan
Nov 1, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·P P Bertrand
May 19, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D FerensJ B Furness
May 19, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D S StrongG M Mawe
Jan 16, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·R Y WuW A Kunze
Oct 1, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B WangW Kunze
Jan 20, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M M HaoH M Young
May 2, 2006·Autonomic Neuroscience : Basic & Clinical·Craig B NeylonJohn B Furness
Apr 28, 2006·American Journal of Physiology. Cell Physiology·Susan Troutman HalmDan R Halm
Jul 4, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Rebecca E HaddockCaryl E Hill
Aug 11, 2006·Journal of Neurophysiology·Yukang MaoWolfgang Kunze
Mar 10, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yaohui ZhuJan D Huizinga
Sep 25, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Timothy J HibberdNick J Spencer
Aug 5, 2017·Frontiers in Physiology·Bob Shih-Liang LeeKirk L Hamilton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.