Intermediate conductance potassium (IK) channels occur in human enteric neurons

Autonomic Neuroscience : Basic & Clinical
John B FurnessJoe J Tjandra

Abstract

IK channels, which had been previously found in hemopoetically derived cells (including erythrocytes and lymphocytes) and epithelial cells, where they regulate proliferation, cell volume regulation and secretion, have only recently been discovered in neurons, where they had previously been claimed not to occur. Based on immunohistochemical detection of IK channel-like immunoreactivity, it has been reported that IK channel expression in enteric neurons is suppressed in Crohn's disease. In the present work we have investigated whether authentic IK channels are expressed by enteric neurons. Human and mouse tissue was investigated by immunohistochemistry, Western blot and RT-PCR. Immunohistochemical studies revealed IK channel-like immunoreactivity in large myenteric neurons, but not in other cell types in the external muscle layers. Many of these nerve cells had calbindin immunoreactivity. Western blots from the external muscle revealed an immunoreactive band at the molecular weight of the IK channel. Using RT-PCR, we detected a transcript corresponding to the IK channel gene in extracts from the ganglion containing layer. The sequence obtained from the RT-PCR product was identical to that previously published for the IK channel. ...Continue Reading

References

Jan 16, 2002·The Journal of Physiology·Fivos VogalisJohn B Furness
May 17, 2002·Progress in Neurobiology·Pankaj Sah, E S Louise Faber
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Jun WangYasunobu Okada
Dec 17, 2002·Current Opinion in Pharmacology·Nadine ClercJohn B Furness
Oct 14, 2003·Pflügers Archiv : European journal of physiology·Mitsuhisa KawaiJohn B Furness

❮ Previous
Next ❯

Citations

Jun 8, 2012·Journal of Signal Transduction·Luigi CatacuzzenoFabio Franciolini
Jan 15, 2014·Pflügers Archiv : European journal of physiology·Ray W Turner, Gerald W Zamponi
May 7, 2014·Pflügers Archiv : European journal of physiology·Ray W TurnerGerald W Zamponi
Apr 21, 2006·Autonomic Neuroscience : Basic & Clinical·Valentina VasinaRoberto De Giorgio
May 2, 2006·Autonomic Neuroscience : Basic & Clinical·Craig B NeylonJohn B Furness
Feb 16, 2006·Autonomic Neuroscience : Basic & Clinical·John B Furness
Feb 8, 2006·Current Opinion in Gastroenterology·David Grundy, Michael Schemann
Aug 11, 2006·Journal of Neurophysiology·Yukang MaoWolfgang Kunze
May 1, 2010·Expert Review of Clinical Pharmacology·Heike Wulff, Neil A Castle
Aug 15, 2018·Frontiers in Molecular Neuroscience·Aravind S KshatriTeresa Giraldez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.