Intermediate Monocytes and Cytokine Production Associated With Severe Forms of Chagas Disease

Frontiers in Immunology
Sergio Gómez-OlarteJohn M González

Abstract

Monocytes are classified according to their CD14 and CD16 expression into classical (reparative), intermediate (inflammatory), and non-classical. This study assessed the frequency of monocyte and the relationship between monocyte subset percentages and the levels of blood cytokines in Colombian chagasic patients with different clinical forms. This study included chagasic patients in different clinical stages: indeterminate (IND) n = 14, chronic chagasic cardiomyopathy (CCC) n = 14, and heart transplant chagasic (HTCC) n = 9; controls with non-chagasic cardiopathy (NCC) n = 15, and healthy individuals (HI) n = 15. Peripheral blood mononuclear cells (PBMCs) were isolated, labeled for CD14, CD16, and HLA-DR, and analyzed by flow cytometry. Cytokines were measured with a bead-based immunoassay. Percentages of total CD14+ CD16+ and CD14+ HLA-DR+ monocytes were higher in patients with heart involvement (CCC, HTCC, and NCC) than controls. Percentages of intermediate monocytes increased in symptomatic chagasic patients (CCC and HTCC) compared to asymptomatic chagasic patients (IND) and controls (HI). Asymptomatic chagasic patients (IND) had higher percentages of classical monocytes, an increased production of CCL17 chemokine compared t...Continue Reading

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Citations

Sep 26, 2020·Frontiers in Cellular and Infection Microbiology·Marcela de Souza-BasqueiraEster Cerdeira Sabino
Jan 27, 2020·European Journal of Clinical Pharmacology·Carolina Pinto VieiraVera Lucia Lanchote
Oct 12, 2020·The Journal of Allergy and Clinical Immunology·Andrzej EljaszewiczMilena Sokolowska
May 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·P VishnyakovaT Fatkhudinov
Jul 3, 2021·International Journal of Molecular Sciences·Mei ElGindiJeremy C M Teo

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Methods Mentioned

BETA
ELISA
density gradient centrifugation
flow cytometry

Software Mentioned

[UNK]
FACSDiva
GraphPad Prism

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