Apr 3, 2020

Intermediate progenitors support migration of neural stem cells into dentate gyrus outer neurogenic niches

ELife
Branden R NelsonRobert Hevner

Abstract

The hippocampal dentate gyrus (DG) is a unique brain region maintaining neural stem cells (NCSs) and neurogenesis into adulthood. We used multiphoton imaging to visualize for genetically defined progenitor subpopulations in live slices across key stages of mouse DG development testing decades old static models of DG formation, with molecular identification, genetic-lineage tracing, and mutant analyses. We found novel progenitor migrations, timings, dynamic cell-cell interactions, signaling activities, and routes underlie mosaic DG formation. Intermediate progenitors (IPs, Tbr2+) pioneered migrations, supporting and guiding later emigrating NSCs (Sox9+) through multiple transient zones prior to converging at the nascent outer adult niche in a dynamic settling process, generating all prenatal and postnatal granule neurons in defined spatiotemporal order. IPs (Dll1+) extensively targeted contacts to mitotic NSCs (Notch active), revealing a substrate for cell-cell contact support during migrations, a developmental feature maintained in adults. Mouse DG formation shares conserved features of human neocortical expansion.

  • References74
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Microscopy, Fluorescence, Multiphoton
Laboratory mice
Dlk1 protein, mouse
Gene Mutant
TbR2-2
NOTCH1
Cell Growth
Stem Cells
Brain Region
Analysis

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Allen Institute for Brain Science Network

The Allen Institute for Brain Science Network is a not-for-profit biomedical research organization that provides open access to multiple neuroscience tools and resources. Find the latest research from tjr Allen Institute for Brain Science Network here.

Related Papers

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Tamar LichtEli Keshet
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Rebecca D HodgeRobert F Hevner
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Hirofumi NoguchiKinichi Nakashima
© 2020 Meta ULC. All rights reserved