Jan 15, 1976

Intermediates in the interconversion of acid and alkaline ferrihemoglobin. Structural and kinetic aspects

European Journal of Biochemistry
B Chance, N M Rumen

Abstract

The acid-alkaline pH-jump in suspension of crystalline sheep hemoglobin has been studied in the range of 5.95 to 8.94. Crystals suspended in 3.8 M Cs2SO4 show a rapid optical transition of half-time equal to or less than 2 ms. As the ammonia concentration is increased in the Cs2SO4-suspended crystals, a second optical transition is observed as a pseudo-first-order reaction, with a rate constant of between 10 and 15 s-1. The alkaline-acid pH-jump proceeds through a very rapid shift of the alkaline-acid equilibrium and is followed by a first-order dissociation constant between 9 and 12 s-1. The dissociation of the ammonia is biphasic, and the ratio between the fast and slow phases is 9.

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Citations

Mentioned in this Paper

Crystal - Body Material
Methemoglobin
Pseudo brand of pseudoephedrine
Dall Sheep
Blood Ammonia Measurement
Ammonia Inhalants
Hemoglobin Measurement
Eryhem
Sheep antigen
Spectrophotometry

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