Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways

Stem Cells International
Xiyao PangJinhua Yu

Abstract

Parathyroid hormone (PTH) is considered to be essential during the tooth development. Stem cells from the apical papilla (SCAPs) are responsible for dentine formation. However, the interaction between PTH and SCAPs remains unclear. This study was aimed at investigating the effects of PTH on odonto/osteogenic differentiation capacity of SCAPs and elucidating the underlying molecular mechanisms. Materials and Methods. Here, SCAPs were isolated and identified in vitro. Effects of PTH on the proliferation of SCAPs were determined by Cell Counting Kit-8 (CCK-8), flow cytometry (FCM), and EdU. Alkaline phosphatase (ALP) activity, alizarin red staining, Western blot, and RT-PCR were carried out to detect the odonto/osteogenic differentiation of PTH-treated SCAPs as well as the participation of the MAPK signaling pathway. An ALP activity assay determined that 10-8 mol/L PTH was the optimal concentration for the induction of SCAPs with no significant influence on the proliferation of SCAPs as indicated by CCK-8, FCM, and EdU. The expression of odonto/osteogenic markers was significantly upregulated in mRNA levels and protein levels. Moreover, intermittent treatment of PTH also increased phosphorylation of JNK and P38, and the differenti...Continue Reading

References

Jun 24, 2005·Journal of Dental Research·S LossdörferA Jäger
Mar 23, 2006·Annual Review of Immunology·Adam Mor, Mark R Philips
May 25, 2007·The Journal of Biological Chemistry·Michael S HuangYin Tintut
Sep 5, 2009·Spine·Stephan SehmischEwa K Stuermer
Sep 29, 2009·Journal of Dental Research·S ChenM MacDougall
Jul 1, 2010·Kidney & Blood Pressure Research·Daniel CejkaMartin Haas
Aug 21, 2013·Journal of Endodontics·Drake W WilliamsReuben H Kim
Aug 27, 2013·Journal of Molecular Histology·Tao XuJinhua Yu
Feb 5, 2014·Journal of Dental Research·A D BakkerW F Lems
Jul 22, 2015·Rheumatology International·Shahryar Noordin, Julie Glowacki
Sep 19, 2015·Cells, Tissues, Organs·Chaitanya P PuranikEric T Everett
Sep 24, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Su Mi WooJi Yeon Jung
Jul 17, 2016·Molecular Neurobiology·Ajay KumarShalmoli Bhattacharyya
Jan 21, 2017·Journal of Dental Research·A KhayatP C Yelick
Apr 4, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Mei HuangSimon C F Rawlinson
Oct 2, 2017·Journal of Clinical Periodontology·Henrique Rinaldi MatheusJuliano Milanezi de Almeida
Nov 14, 2017·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Jie LiuHua Tang
Nov 16, 2017·International Journal of Molecular Medicine·Xiao YinLongxing Ni
Dec 1, 2017·Journal of Dental Research·V C M Neves, P T Sharpe

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BETA
flow cytometry
PCR
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