Intermittent anoxia induces oxidative stress in wheat seminal roots: assessment of the antioxidant defence system, lipid peroxidation and tissue solutes

Functional Plant Biology : FPB
Danica E Goggin, Timothy D Colmer

Abstract

The effects of continuous and intermittent anoxia on components of the antioxidant defence system were evaluated in the expanded zones of wheat seedling roots. Intermittent anoxia caused oxidative stress (measured by the proportion of reduced glutathione) after three cycles of anoxia-aeration. The concentration of glutathione and activities of glutathione reductase (GR) and catalase (CAT) were decreased by 50% under both continuous and intermittent anoxia. Ascorbate peroxidase (APX) activity was unaffected by anoxia but stimulated almost 2-fold during the aerated periods of intermittent anoxia. Superoxide dismutase activity was decreased by 20% under continuous anoxia but ultimately returned to aerated activities under intermittent anoxia. Membrane damage appeared to be negligible or reversible, as K+ concentrations recovered to original levels under intermittent anoxia and there was no increase in terminal lipid peroxidation products. Addition of 5 mm exogenous ascorbate to intermittently anoxic roots prevented oxidative stress and avoided the decreases in glutathione, GR and CAT. Therefore, it is likely that the oxidative stress resulted from inadequate levels of, or damage to, these two enzymes.

References

Jul 18, 1996·Biochimica Et Biophysica Acta·O M LardinoisP G Rouxhet
Jan 2, 2003·Annals of Botany·Olga BlokhinaKurt V Fagerstedt
Sep 19, 2003·Journal of Bioenergetics and Biomembranes·Marcos BrandalisePaulo Arruda
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Malcolm C. Drew
Mar 1, 2003·Functional Plant Biology : FPB·Jane Gibbs, Hank Greenway

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Citations

Nov 14, 2015·Plant, Cell & Environment·Max HerzogOle Pedersen
Aug 1, 2009·Functional Plant Biology : FPB·T D Colmer, L A C J Voesenek

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Methods Mentioned

BETA
Protein Assay

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