Intermittent food restriction in female rats induces SREBP high expression in hypothalamus and immediately postfasting hyperphagia

Nutrition
Mariana Alejandra Rosas FernándezKelse Tibau de Albuquerque

Abstract

We investigated the effect of intermittent food restriction (IFR) cycles on hypothalamic expression of lipogenic proteins and induction of overeating. Female Wistar rats were distributed in three groups: free access to feed (control, C), 2 d feed restriction at 50% of C intake followed by 3 d (restricted 3, R3) or 5 d (restricted 5, R5) ad libitum feeding. After 6 wk, the rats were submitted to euthanasia and collected the hypothalamus and blood. The deposits of retroperitoneal, mesenteric, and gonadal fat were weighed. The expression of the mRNA for sterol regulatory element binding protein (SREBP) 1c and 2 and acetyl-CoA carboxylase in the hypothalamus were determined by real-time polymerase chain reaction, and glucose and triacylglycerol were evaluated by a commercial kit. Body mass and food intake were measured daily. IFR promoted increased expression of SREBP-2 in both treated groups and, in R5, increased expression of SREBP-1c. The serum triacylglycerol, mesenteric deposit, and total fat content were higher in R3. Neither of the treatment intervals altered the expression of the mRNA of acetyl-CoA carboxylase enzyme but induced hyperglycemia and higher food intake immediately after food restriction. IFR affected the expres...Continue Reading

Citations

Jun 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Rachel J PerryGerald I Shulman
Oct 16, 2020·The British Journal of Nutrition·Mariana Rosas FernándezKelse T Albuquerque

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