Intermittent hydrostatic pressure maintains and enhances the chondrogenic differentiation of cartilage progenitor cells cultivated in alginate beads

Development, Growth & Differentiation
Yang LiJianchao Gui

Abstract

The objective of this study was to explore the effects of intermittent hydrostatic pressure (IHP) on the chondrogenic differentiation of cartilage progenitor cells (CPCs) cultivated in alginate beads. CPCs were isolated from the knee joint cartilage of rabbits, and infrapatellar fat pad-derived stem cells (FPSCs) and chondrocytes (CCs) were included as the control cell types. Cells embedded in alginate beads were treated with IHP at 5 Mpa and 0.5 Hz for 4 h/day for 1, 2, or 4 weeks. The cells' migratory and proliferative capacities were evaluated using the scratch and Live/Dead assays, respectively. Hematoxylin and eosin staining, safranin O staining, and immunohistochemical staining were performed to determine the effects of IHP on the synthesis of extracellular matrix (ECM) proteins. Real-time polymerase chain reaction analysis was performed to measure the expression of genes related to chondrogenesis. The scratch and Live/Dead assays revealed that IHP significantly promoted the migration and proliferation of FPSCs and CPCs to different extents. The staining experiments showed greater production of cartilage ECM components (glycosaminoglycans and collagen II) by cells exposed to IHP, and the gene expression analysis demonstra...Continue Reading

References

Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Apr 2, 1999·Science·M F PittengerD R Marshak
Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Takashi IkenoueR Lane Smith
Apr 23, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·P AngeleB Johnstone
Feb 6, 2004·Journal of Cell Science·Gary P DowthwaiteCharles W Archer
Mar 1, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Eric M Darling, Kyriacos A Athanasiou
Apr 1, 2006·Advanced Drug Delivery Reviews·Dobrila NesicPierre Mainil-Varlet
May 8, 2007·Biochemical and Biophysical Research Communications·Shintaro HattoriA Hari Reddi
May 28, 2008·Injury·Carl HaasperMichael Jagodzinski
Feb 7, 2009·Tissue Engineering. Part B, Reviews·Benjamin D Elder, Kyriacos A Athanasiou
Mar 31, 2009·Trends in Biotechnology·Claire VinatierDanièle Noël
Feb 22, 2012·Journal of Materials Science. Materials in Medicine·Monica L MoyaEric M Brey
Mar 1, 2012·European Cells & Materials·Tatiana VinardellDaniel J Kelly
Apr 12, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jong Min LeeGun-Il Im

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Citations

Jun 14, 2018·Tissue Engineering. Part B, Reviews·Mingxue ChenQuanyi Guo
Apr 10, 2019·Journal of Tissue Engineering and Regenerative Medicine·Takahiro OguraShuichi Mizuno
May 10, 2017·Journal of Tissue Engineering and Regenerative Medicine·Jessica SchiaviCéline Huselstein
Dec 15, 2020·Journal of Cell Science·Koichiro MakiSara A Wickström
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Sarah DavisTosca Roncada
Aug 26, 2021·Journal of Cell Science·Koichiro MakiSara A Wickström

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