Intermittent Pringle maneuver is unlikely to induce bacterial translocation to the portal vein: a study using bacterium-specific ribosomal RNA-targeted reverse transcription-polymerase chain reaction

Journal of Hepato-biliary-pancreatic Sciences
Naoya YamaguchiMasato Nagino

Abstract

The occurrence of bacterial translocation (BT) to the mesenteric lymph nodes following the Pringle maneuver is well established; however, the incidence of BT to the portal circulation remains unclear. Portal blood of patients with suspected hilar malignancy who underwent major hepatobiliary resection with cholangiojejunostomy was sampled three times during surgery: immediately after laparotomy (PV-1); before liver transection and after skeletonization of the hepatoduodenal ligament (PV-2); and after completion of the liver transection (PV-3). The samples were analyzed for microbes with a bacterium-specific ribosomal RNA-targeted reverse transcription-polymerase chain reaction method. Fifty patients were enrolled in the study, with a mean total Pringle time of 86 min. Microbes in the portal blood were detected in 11 (22%) of the 50 patients. The occurrence of microbes was not different among the PV-1 samples (8% = 4/50), PV-2 samples (14% = 7/50), and PV-3 samples (14% = 7/50) (P = 0.567). Obligate anaerobes were predominantly detected. The positivity of the PV-3 samples showed no correlation with the total Pringle time or with the occurrence of postoperative infectious complications. The total Pringle time did not affect the su...Continue Reading

References

Jun 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G Garcia-TsaoA B West
Jul 1, 1996·Applied and Environmental Microbiology·K H Wilson, R B Blitchington
Aug 13, 2002·Langenbeck's Archives of Surgery·Hidetaka ShigetaYuji Nimura
Jul 10, 2003·The Journal of Surgical Research·Dah-Cherng YehFang-Ku P'eng
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Jul 19, 2006·Archives of Surgery·Yoichi IshizakiSeiji Kawasaki
Oct 24, 2006·The British Journal of Surgery·M NaginoY Nimura
Oct 31, 2006·Applied and Environmental Microbiology·Kazunori MatsudaKoji Nomoto
Oct 1, 1908·Annals of Surgery·J H Pringle
Mar 31, 2010·Journal of Clinical Microbiology·Sachi SakaguchiToshiaki Shimizu
Jul 16, 2010·The British Journal of Surgery·Y SugiyamaS Kawasaki
Oct 26, 2010·Digestive Diseases and Sciences·Petros YpsilantisConstantinos Simopoulos
Aug 1, 2012·Journal of Gastroenterology·Masato Nagino

❮ Previous
Next ❯

Citations

Feb 6, 2016·Archives of Trauma Research·Mohsen KolahdoozanMohammad Taghi Rezaei
Dec 15, 2020·Journal of the American College of Surgeons·Masaya SuenagaYasuhiro Kodera

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.