Intermittent reloading attenuates muscle atrophy through modulating Akt/mTOR pathway

Medicine and Science in Sports and Exercise
Mitsunori MiyazakiTohru Takemasa

Abstract

The aim of this study is to investigate the effects of intermittent reloading during hindlimb unloading (HU) on the changes in intracellular signaling pathways in skeletal muscle. Male Wister rats were divided randomly into one of three experimental groups: 1) nonsuspended control, 2) HU for 7 d, and 3) HU with intermittent reloading (HU/IR) for 4 h.d(-1). After each experimental period, the antigravitational soleus muscle was analyzed. After 7 d of HU treatment, muscle fiber atrophy (decrease in relative muscle mass: 0.28 mg.g(-1) in the HU group vs 0.36 mg.g(-1) in the control group, P < 0.05; decrease in fiber CSA: 1682.6 microm2 in the HU group vs 2673.0 microm2 in the control group, P < 0.05) and a decrease in phosphorylation levels of anabolic signaling pathway (Akt and mTOR) were observed. Additionally, expressions of two types of muscle-specific E3 ubiquitin ligase mRNA (muscle atrophy F-box (MAFbx), and muscle ring finger 1 (MuRF1)) were upregulated during muscle atrophy. Increases in binding activities of nuclear factor kappa B (NFkappaB) were also determined. In contrast, IR treatment attenuated the muscle fiber atrophy (0.33 mg.g(-1) and 2067.5 microm2) and partially increased the phosphorylation levels of anabolic ...Continue Reading

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Citations

Jan 9, 2009·American Journal of Physiology. Cell Physiology·Mitsunori Miyazaki, Karyn A Esser
Mar 10, 2011·BMC Musculoskeletal Disorders·Parco M SiuEric W Tam
Apr 19, 2012·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Olasunkanmi A J AdegokePegah Tavajohi-Fini
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Jan 25, 2017·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Yusuke HirayamaHidemi Fujino
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May 30, 2020·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Paul T ReidyMicah J Drummond
Mar 18, 2020·Frontiers in Nutrition·Shinya Aoyama, Shigenobu Shibata
Nov 25, 2020·Annals of the New York Academy of Sciences·Apratim BajpaiWeiqiang Chen

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