PMID: 6413989Jul 1, 1983Paper

Intermolecular recombination during transformation of Bacillus subtilis competent cells by monomeric and dimeric plasmids

Plasmid
B MichelS D Ehrlich

Abstract

Bacillus subtilis competent cells harboring plasmid pUB110 were transformed by plasmids unable to replicate in this host but carrying segments of pUB110, 260 to 4500 bp long. Recombinants between the incoming and the resident plasmids were found in the transformed cells. Transforming efficiency of the incoming plasmids depended strongly on their molecular form and the length of their region homologous with the resident plasmid. It increased with the fourth to fifth power of that length for monomers having at least 900 bp of homology. Activity of monomers having less than 900 bp homology was too low to be measured in our experiments. Transforming efficiency of dimers was much greater than that of monomers, and varied with the square of the length of the homologous region. These results indicate that dimeric and monomeric plasmid molecules are processed differently during transformation of B. subtilis competent cells.

References

Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S D Ehrlich
Sep 1, 1981·Plasmid·S B Primrose, S D Ehrlich

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Citations

Sep 1, 1986·Molecular & General Genetics : MGG·B VosmanG Venema
May 1, 1986·Molecular & General Genetics : MGG·J H Stuy, R B Walter
Mar 1, 1990·Gene·L JannièreS D Ehrlich
Jul 5, 1987·Journal of Molecular Biology·P NoirotS D Ehrlich
Dec 4, 2012·Journal of Microbiological Methods·Sai WenTianwei Tan
Jun 28, 2002·The Journal of Biological Chemistry·Anna PluciennikRobert D Wells
Jun 1, 1989·Journal of Bacteriology·L BoeA Gruss
Feb 1, 1989·Applied and Environmental Microbiology·K J LeenhoutsG Venema
Sep 1, 1991·Applied and Environmental Microbiology·K J LeenhoutsG Venema
Sep 1, 1990·Applied and Environmental Microbiology·K J LeenhoutsG Venema
Sep 1, 1991·Microbiological Reviews·D Dubnau

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