PMID: 6980263Jun 1, 1982Paper

Internal and external differentiations of the postsynaptic membrane at the neuromuscular junction

Journal of Neurocytology
N Hirokawa, J E Heuser

Abstract

Frog, snake and rat neuromuscular junctions were prepared for electron microscopy by the quick-freeze, deep-etch, rotary replication procedure. The postsynaptic membrane was exposed by treating muscles with 1 mg/ml collagenase to remove the basal lamina. Present on the apices of the postsynaptic folds are regular arrays of 8-9 nm protrusions. These are not seen in the depths of the folds nor elsewhere on the muscle surface, thus they presumably represent the heads of cholinergic receptor molecules. These protrusions tend to be arranged in parallel rows two-abreast. Their high concentration (10 000/microns2) and their orderly arrangement is basically similar to the receptors seen in Torpedo postsynaptic membrane. Their distribution did not appear to change after denervation. Efforts were made to expose possible anchoring structures of these receptors, by treating muscles with 0.1% Saponin immediately before and/or during fixation in 1% formaldehyde, or by homogenizing muscles after brief formaldehyde fixation. This washed most soluble protein out of the cytoplasm and exposed a submembraneous meshwork just beneath the postsynaptic membrane. This meshwork appears to connect the membrane to underlying bundles of intermediate filame...Continue Reading

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Citations

Jun 1, 1984·Cellular and Molecular Neurobiology·B MéndezN C Inestrosa
Jun 1, 1990·Cellular and Molecular Neurobiology·J IovannaD P Cardinali
Oct 1, 1983·Journal of Neurocytology·J Ross-CanadaG D Pappas
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