Internal initiation of mRNA translation in insect cell mediated by an internal ribosome entry site (IRES) from shrimp white spot syndrome virus (WSSV)

Biochemical and Biophysical Research Communications
Fang Han, Xiaobo Zhang

Abstract

Internal initiation of mRNA translation can be mediated by internal ribosome entry site (IRES) elements which are located mainly in RNA viruses as well as certain mammalian and insect mRNA molecules. Thus far, only one DNA virus has been discovered to contain IRES element. In this investigation, an IRES element from white spot syndrome virus (WSSV), a DNA virus of marine shrimp, was demonstrated to direct the efficient translation of dicistronic mRNA in Trichoplusia ni insect cells. The IRES was inserted between glutathione S-transferase (GST) and green fluorescent protein (GFP) genes to construct a dicistronic cassette (GST-IRES-GFP). After transfection of this dicistronic cassette in insect cell, the Northern blot indicated that only one transcript corresponding to the mRNA of GST-IRES-GFP could be detected. However, the GST and GFP genes were simultaneously translated as revealed by Western blot and fluorescent microscopy, respectively. Based on sequence orientation and deletion analyses, the IRES element was 180 nucleotides (nt) in length and orientation-dependent. By comparison with that of cap-dependent initiation, the translation efficiency mediated by IRES was 98.77%. This finding promises that the WSSV IRES could be ve...Continue Reading

References

Feb 1, 1989·The Journal of Cell Biology·M Kozak
Aug 1, 1997·Journal of Virological Methods·F YangX Xu
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·S A ChappellV P Mauro
Aug 9, 2005·Biochemical and Biophysical Research Communications·Ying-Ju ChenTzong-Yuan Wu
Sep 16, 2005·Nature·Curtis A Suttle

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Citations

Nov 16, 2012·Journal of Biosciences·Sumit Chowdhury, Bhaskar Sharma
Dec 18, 2007·Trends in Microbiology·Bert L Semler, Marian L Waterman
Jul 28, 2007·Virology·Deng-Feng LiXun Xu
Sep 5, 2012·Trends in Microbiology·Sunnie R Thompson
Sep 22, 2016·Critical Reviews in Biochemistry and Molecular Biology·Marni S CrowIleana M Cristea

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