Internal motion of lysozyme studied by time-resolved fluorescence depolarization of tryptophan residues

Biochemistry
Etsuko NishimotoT Imoto

Abstract

The internal motion of lysozyme was described by the steady-state and time-resolved fluorescence anisotropy of its tryptophan residues. The fluorescence of mutant lysozymes W62Y- and W108Y-lysozyme, in which Trp62 or Trp108 of hen egg white lysozyme was replaced with a tyrosine residue, could be respectively assigned to Trp108 or Trp62 at the longer wavelength region of the total fluorescence spectrum. The segmental motion of Trp62 as shown by its fluorescence anisotropy decay was described with two components originating from the fluctuational rotation of an indole moiety about the Calpha-Cbeta bond and rotational wobble of the peptide segment adjacent to Trp62. Although Trp62 showed a high degree of motional freedom, its motion was significantly suppressed by the interaction of the mutant protein with a trimer of N-acetyl-D-glucosamine. By contrast, the segmental motion of Trp108 is hindered by the local cage structure at temperatures below 30 degreesC, but Relief from restricted motion occurred on the formation of ligand complex or by thermal agitation. Because of overlaps of the fluorescence spectrum, it is difficult to assign the segmental motion of Trp28 or Trp111, the other two tryptophan residues in lysozyme. However, a...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·I MunroL Stryer
Sep 1, 1986·Biophysical Journal·A J Cross, G R Fleming
Sep 29, 1995·Journal of Molecular Biology·P H HünenbergerW F van Gunsteren

❮ Previous
Next ❯

Citations

Jul 8, 2008·Journal of Biochemistry·Yukihiro FukunagaShoji Yamashita
Jun 8, 2001·Alcoholism, Clinical and Experimental Research·S D ShuklaJ B Hoek
Jun 10, 2008·Bioscience, Biotechnology, and Biochemistry·Daisuke TakahashiShoji Yamashita
Mar 19, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jian CaoFuguang Jiang
Oct 17, 2012·Chemistry and Physics of Lipids·Galyna GorbenkoPaavo Kinnunen
May 21, 2011·Journal of Photochemistry and Photobiology. B, Biology·Hong-Mei ZhangYan-Qing Wang
Jan 23, 2010·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yan-Qing WangHong-Mei Zhang
Jun 23, 2001·Journal of Molecular Biology·N TaschnerT Arvinte
Apr 17, 2007·Biophysical Journal·Galyna P GorbenkoPaavo K J Kinnunen
Aug 27, 2015·Soft Matter·Yang SunCristiano L P Oliveira
Sep 28, 2015·Journal of Fluorescence·Michael V RusalovMichael V Alfimov
Mar 4, 2008·Biophysical Journal·Daisuke TakahashiShoji Yamashita
Nov 29, 2014·Biopolymers·Jeremiah J Babcock, Lorenzo Brancaleon
Aug 6, 2010·Methods : a Companion to Methods in Enzymology·Sarah A WeinreisSilvia Cavagnero
Oct 30, 2007·Biochimica Et Biophysica Acta·Galyna P GorbenkoPaavo K J Kinnunen
Mar 4, 2017·The Journal of Physical Chemistry. B·Pegah S Nabavi Zadeh, Björn Åkerman
Jul 25, 2006·Journal of Biochemistry·Etsuko NishimotoShoji Yamashita
Nov 30, 2002·The Journal of Biological Chemistry·Soon-Jong KimSamuel H Wilson
Oct 14, 2009·Molecular Biology Reports·Hong-Mei ZhangYan-Qing Wang
Jul 3, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Yanqiu ZuoZhongsheng Yi
Jul 28, 2009·Analytical Chemistry·Josephine RutaEric Peyrin
May 12, 2019·International Journal of Biological Macromolecules·Ali ChaariMohamed Rholam
Mar 4, 2009·The Journal of Physical Chemistry. a·Takuhiro OtosuShoji Yamashita
Aug 12, 2019·Colloids and Surfaces. B, Biointerfaces·Mbaye NdourSebastien Balme
Sep 8, 2011·The Journal of Physical Chemistry. B·Daisuke TakahashiEtsuko Nishimoto
Nov 14, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Yurij A AntonovRuth Cardinaels
Sep 19, 2012·The Journal of Physical Chemistry. B·Samuele RaccostaMauro Manno

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.