Internal validation of STRmix™ for the interpretation of single source and mixed DNA profiles

Forensic Science International. Genetics
T R MorettiAnthony J Onorato

Abstract

The interpretation of DNA evidence can entail analysis of challenging STR typing results. Genotypes inferred from low quality or quantity specimens, or mixed DNA samples originating from multiple contributors, can result in weak or inconclusive match probabilities when a binary interpretation method and necessary thresholds (such as a stochastic threshold) are employed. Probabilistic genotyping approaches, such as fully continuous methods that incorporate empirically determined biological parameter models, enable usage of more of the profile information and reduce subjectivity in interpretation. As a result, software-based probabilistic analyses tend to produce more consistent and more informative results regarding potential contributors to DNA evidence. Studies to assess and internally validate the probabilistic genotyping software STRmix™ for casework usage at the Federal Bureau of Investigation Laboratory were conducted using lab-specific parameters and more than 300 single-source and mixed contributor profiles. Simulated forensic specimens, including constructed mixtures that included DNA from two to five donors across a broad range of template amounts and contributor proportions, were used to examine the sensitivity and sp...Continue Reading

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Citations

Feb 23, 2018·International Journal of Legal Medicine·Jennifer D ChurchillBruce Budowle
Aug 23, 2018·Journal of Forensic Sciences·John S BuckletonDuncan A Taylor
May 29, 2018·International Journal of Legal Medicine·Ruiyang TaoChengtao Li
Nov 4, 2020·Forensic Science International. Genetics·John BuckletonJames Curran
Dec 29, 2020·Science & Justice : Journal of the Forensic Science Society·Kaitlin HuffmanJack Ballantyne
Jan 2, 2021·Forensic Science International : Synergy·John M Butler, Sheila Willis
Nov 23, 2019·Forensic Science International. Genetics·Lisa DierigPeter Wiegand
Jun 3, 2018·Forensic Science International : Synergy·Simone GittelsonJohn S Buckleton
Mar 12, 2018·Forensic Science International. Genetics·Rebecca S Just, Jodi A Irwin
Jul 6, 2021·Forensic Science International. Genetics·Amy GriffinAdrian Linacre
Sep 3, 2021·Forensic Science International. Genetics·Max SchmidtVolker Weirich

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