Internalization and transcytosis of pancreatic enzymes by the intestinal mucosa

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Maryse CloutierMoise Bendayan

Abstract

As early as the beginning of the twentieth century some data indicated that macromolecules are able to cross the intestinal mucosa to reach the blood. Further evidence was added over the years; however, pathways for this transport still remain to be established. We report here the transfer of two pancreatic enzymes, amylase and lipase, from the intestinal lumen to the blood. Both are present in higher concentrations in the intestinal mucosa and in blood of fed rats. Upon cholinergic stimulation of pancreatic secretion, there was not only an increase in blood enzyme concentrations, but evidence for internalization by duodenal enterocytes was obtained. Following insertion of fluorochrome-tagged amylase and lipase into the duodenal lumen of fasting rats, blood and intestinal tissues were sampled at different time points. Serum activities for both enzymes clearly increased with time. Light microscopy established internalization of both proteins by duodenal enterocytes, and immunogold outlined the pathway taken by both proteins across the enterocytes. From the intestinal lumen, enzymes are channeled through the endosomal compartment to the Golgi apparatus and to the basolateral membrane reaching the interstitial space and blood circ...Continue Reading

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Citations

May 11, 2010·International Journal of Cell Biology·Philippe G CammisottoEmile Levy
May 2, 2009·Cell and Tissue Research·Flavia MerigoAndrea Sbarbati
Jul 14, 2010·Progress in Histochemistry and Cytochemistry·Philippe G CammisottoMoise Bendayan
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Apr 24, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Krzysztof GolabMaria Warwas
Sep 19, 2016·Current Diabetes Reports·Catherine Peyrot des Gachons, Paul A S Breslin
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Apr 27, 2012·Anatomy & Cell Biology·Philippe Cammisotto, Moise Bendayan
Mar 13, 2020·Histochemistry and Cell Biology·Irina S SesorovaAlexander A Mironov
Dec 1, 2008·Expert Review of Respiratory Medicine·John A Dodge
Aug 4, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Philippe G CammisottoMoïse Bendayan

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Methods Mentioned

BETA
light microscopy
electrophoresis
electron microscopy
light
glycosylation

Software Mentioned

Videoplan

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