PMID: 2119144Sep 1, 1990Paper

Internalization of fluorescent vasotocin-receptor agonist and antagonist in the toad bladder

The American Journal of Physiology
P EggenaA Buku

Abstract

This study compares hydrosmotic action, receptor binding, and fluorescent uptake of an agonist, d9phe(flu)AVT, and an antagonist, d4lys(flu)AVT, in the toad bladder. D9phe(flu)AVT increased osmotic water flow across the bladder with a 50% effective dose of 2 nM, whereas d4lys(flu)AVT inhibited water flow with a 50% effective dose of 0.1 microM. D9phe(flu)aVT displaced 10 nM [3H]arginine vasopressin (AVP) from plasma membranes by 50% (IC50) with 10 nM, whereas d4lys(flu)AVT had an IC50 of 3 microM. The fluorescent agonist induced a persistent increase in membrane permeability to water after removal from the serosal bathing solution. This residual response was diminished by preincubation with an agonist (AVP), but not with an antagonist [d4lys(N3)AVT]. The agonist, d9phe(flu)AVT, was internalized into toad bladder epithelial cells, as seen by epifluorescence microscopy, and this uptake was blocked by d4lys(N3)AVT. The antagonist, by contrast, was not internalized but remained at the cell surface. After stimulation with forskolin, however, the fluorescent antagonist was also internalized. These experiments suggest that agonists, but not antagonists, form functional complexes with receptors that, via formation of cAMP, trigger not ...Continue Reading

References

Jan 1, 1986·The Journal of Membrane Biology·S K MasurM S Rubin
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K L KirkP Eggena
May 1, 1967·The American Journal of Medicine·W H Sawyer
Aug 1, 1961·The Journal of Biophysical and Biochemical Cytology·L D PEACHEY, H RASMUSSEN
Dec 1, 1969·The Journal of Membrane Biology·D R DibonaA Leaf

❮ Previous
Next ❯

Citations

Dec 1, 1992·Digestive Diseases and Sciences·R A Erickson, N Rivera
Jun 1, 1995·Journal of Gastroenterology·J StachuraW Domschke
Mar 4, 1998·British Journal of Pharmacology·J F BrownB L Tepperman
Apr 2, 1999·Alimentary Pharmacology & Therapeutics·H HiraishiA Terano
Apr 2, 1999·Alimentary Pharmacology & Therapeutics·L R FitzpatrickT Le
Jan 10, 2001·Alimentary Pharmacology & Therapeutics·H MizoguchiK Takeuchi
Jun 27, 2002·Japanese Journal of Pharmacology·Shinichi Kato, Koji Takeuchi
Aug 13, 2003·Journal of Gastroenterology and Hepatology·Neena Kalia, Karna Dev Bardhan
Sep 1, 2004·Alimentary Pharmacology & Therapeutics·M Lazzaroni, G Bianchi Porro
Dec 8, 2006·Naunyn-Schmiedeberg's Archives of Pharmacology·Cristina PozzoliGabriella Coruzzi
Jan 9, 2008·Journal of Biomedical Science·Tarek K MotawiNancy N Shahin
May 16, 2008·International Endodontic Journal·M YamasakiH Nakamura
May 1, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Antoniella Souza GomesMarcellus Henrique Loiola Ponte Souza
Sep 26, 2015·Digestive Diseases and Sciences·Nathalia S CarvalhoJand Venes R Medeiros
Jun 2, 2016·Digestive Diseases and Sciences·Lee-Shuan LinManabu Mochizuki
Nov 1, 1995·Digestive Diseases and Sciences·N YoshidaM Kondo
Sep 1, 1993·Scandinavian Journal of Gastroenterology·J W KonturekW Domschke
Mar 11, 1996·FEBS Letters·D LapennaF Cuccurullo
Feb 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J R AvilaJ Herrerias
Jun 1, 1995·Inflammation·N YoshidaP R Kvietys
Jun 17, 1999·The New England Journal of Medicine·M M WolfeG Singh
May 12, 2000·Proceedings of the Society for Experimental Biology and Medicine·Y FujiiK Yamada
Apr 24, 1999·Alimentary Pharmacology & Therapeutics·K MurakamiH Okabe
Nov 1, 1995·Digestive Diseases and Sciences·N OkabeA Kuroiwa
Jan 16, 1998·Journal of Gastroenterology·J S Antoon, M A Perry
Jan 29, 2000·Alimentary Pharmacology & Therapeutics·T M NgD Machin
Mar 10, 2001·Free Radical Research·T Yoshikawa, Y Naito

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.