PMID: 6405054Mar 1, 1983Paper

Internalization of iron-transferrin complex by murine L1210 leukemia cells and rat reticulocytes demonstrated by a minibead probe

Journal of Ultrastructure Research
K Takahashi, M Tavassoli

Abstract

To determine if the cellular uptake of iron is associated with internalization of iron-transferrin (TF) complex by the cell, we synthesized a visual probe in which TF is covalently bound to amide-modified latex minibead, submicrometer in size (0.345 micron). Incubation of the probe with L1210 leukemia cells and rat reticulocytes led to the binding of the probe to the cell surface visualized and semiquantified by scanning and transmission electron microscopy. The binding was inhibited by preincubation with nonderivatized iron-TF complex. Internalization of the probe occurred through clathrin-coated pits and vesicles. Minibeads derivatized by nontransport proteins or glycine as well as nonderivatized minibeads did not appreciably bind to the cells and were not internalized. Ethylamine, an inhibitor of receptor-mediated endocytosis abolished the internalization but not the binding of the probe which, then, accumulated on the cell surface. These findings provide direct evidence for internalization of TF during the iron uptake.

References

Jun 21, 1979·Nature·J L GoldsteinM S Brown
May 4, 1978·Biochimica Et Biophysica Acta·M T NunezS H Robinson
May 1, 1977·British Journal of Haematology·D Hemmaplardh, E H Morgan
Nov 27, 1974·Biochimica Et Biophysica Acta·D Hemmaplardh, E H Morgan
Dec 1, 1972·British Journal of Haematology·M TavassoliW H Crosby
Apr 21, 1972·Biochimica Et Biophysica Acta·J Martinez-Medellin, H M Schulman
May 1, 1981·Journal of Ultrastructure Research·K Takahashi, M Tavassoli
Jan 1, 1980·Annual Review of Biochemistry·P Aisen, I Listowsky
Feb 8, 1982·Biochimica Et Biophysica Acta·K Takahashi, M Tavassoli
Mar 1, 1963·The Journal of Clinical Investigation·J H JANDL, J H KATZ
Sep 24, 1963·Biochimica Et Biophysica Acta·R AASAP SALTMAN

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Citations

Aug 14, 1998·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M KresseW Semmler
Nov 1, 1986·The American Journal of Anatomy·S Irie, M Tavassoli
Aug 3, 2018·Nature Communications·Duverney Chaverra-RodriguezJason L Rasgon
Jan 1, 1985·The International Journal of Biochemistry·C G Morley, A Bezkorovainy
Aug 14, 1987·Biochemical and Biophysical Research Communications·T KishimotoD W Jacobsen

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