PMID: 8583242Oct 1, 1995Paper

Internalization of microbubbles by tumor cells in vivo and in vitro

Journal of Neuro-oncology
E BarbareseR H Simon

Abstract

Lipid-coated microbubbles (LCM) administered intravenously (i.v.) to rats bearing brain tumor, specifically enhance tumor visualization by ultrasound [1]. In order to understand the basis for this observation, we have examined the interactions of LCM with glioblastoma (C6) and gliosarcoma (9L) tumor cells in vivo and in vitro. LCM and LCM labeled with the fluorescent lipophilic dye 3,3'-dioctadecyloxacarbocyanine perchlorate (diO) were administered to rats bearing brain tumor. LCM and diO-labeled LCM were found principally at the tumor site with no evidence of label in the surrounding normal brain tissue. Analysis of the tumor by confocal laser scanning microscopy revealed that labeled LCM were inside the tumor cells. Similar analysis of LCM interactions with C6 and 9L cells in culture showed that LCM first adsorb at the surface of the cells, and with time became localized inside the cells. Binding and internalization proceeded faster at 37 degrees C than at room temperature (RT). Staining of live cells with N-(3-((2,4-dinitrophenyl)amino)propyl)-N-(3-aminopropyl) methylamine dihydrochloride (DAMP), a dye that recognizes acidic compartments, showed that the majority of internalized LCM was associated with compartments containin...Continue Reading

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Citations

Jan 1, 1997·Brain Research Bulletin·S Y HoR H Simon
Jan 5, 2011·PloS One·Vladislav TsipersonBridget Shafit-Zagardo
Dec 2, 2004·The British Journal of Radiology·H-D Liang, M J K Blomley
Jul 3, 2013·Pharmacological Reviews·George J ChristKarl-Erik Andersson
Sep 21, 2007·Expert Opinion on Drug Delivery·Joseph L Bull
Jul 6, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Ahmet ErtenMilan Makale
Dec 22, 2007·Advanced Drug Delivery Reviews·Panayiotis P ConstantinidesRobert Shorr
Dec 2, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Grace H HuynhFrancis C Szoka
Nov 22, 2005·Expert Opinion on Drug Delivery·William G PittBryant J Staples
Jan 6, 2004·Journal of Biomedical Materials Research. Part a·T RizkP Menei
Sep 28, 2002·Journal of Magnetic Resonance Imaging : JMRI·Paul A Dayton, Katherine W Ferrara
Apr 24, 1999·Journal of Advanced Nursing·M Nolan, J Nolan
Jun 17, 2005·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S J Gwyther

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