Internalization of Staphylococcus aureus by bovine endothelial cells is associated with the activity state of NF-kappaB and modulated by the pro-inflammatory cytokines TNF-alpha and IL-1beta

Scandinavian Journal of Immunology
Javier Oviedo-BoysoVictor M Baizabal-Aguirre

Abstract

Bacterial internalization is an important process in the pathogenesis of infectious diseases in which nuclear factor kappaB (NF-kappaB) plays a prominent role. We present pharmacological evidence indicating that in bovine endothelial cells (BEC) the internalization of Staphylococcus aureus, a pathogenic bacterium that causes mastitis in bovine cattle, was associated with the activation of NF-kappaB. The internalization of S. aureus increased when BEC were stimulated with alpha-tumour necrosis factor (TNF-alpha) or beta-interleukin 1 (IL-1beta) which are known activators of NF-kappaB. SN50 (an inhibitor peptide of NF-kappaB nuclear translocation) and BAY 11-7083 (a chemical that inhibits the IkappaBalpha phosphorylation) caused significant reduction in S. aureus intracellular number, indicating that its internalization was associated with the NF-kappaB activity. Furthermore, specific inhibition of c-Jun N-terminal kinase with SP600125 (SP) or p-38 with SB203580 (SB) did not cause any change in the S. aureus intracellular number compared with the untreated control. Finally, TNF-alpha treatment of BEC after the addition of both SP and SB, induced a significant increase in S. aureus internalization above the control value. These da...Continue Reading

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Citations

Feb 7, 2013·World Journal of Microbiology & Biotechnology·M H Fabres-KleinA O B Ribon
Jun 21, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M H Fabres-KleinA O B Ribon
Feb 6, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guanghui LiuAnna M Blom
Sep 11, 2008·Clinical and Experimental Immunology·F M TeixeiraH C Teixeira
Feb 5, 2009·FEMS Microbiology Letters·Javier Oviedo-BoysoVíctor M Baizabal-Aguirre
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Nov 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xin WangAizhong Zhang

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