Internalization of the 180 kDa bullous pemphigoid antigen as immune complexes in basal keratinocytes: an important early event in blister formation in bullous pemphigoid

The British Journal of Dermatology
Y KitajimaK Owaribe

Abstract

We have previously shown that the 180 kDa bullous pemphigoid antigen (BPAG2) is distributed on the lateral-apical (as a pool) and ventral (as hemidesmosomes) cell membranes of monolayer cultured keratinocytes and that addition of IgG purified from bullous pemphigoid (BP) patients (BP-IgG) causes the internalization of immune complexes of BPAG2 and BP-IgG from the lateral-apical cell membrane. This internalization of BPAG2 is believed to inhibit the Ca2+ induced reformation of hemidesmosomes on the ventral cell membrane, possibly by inhibiting the supply of the antigen from the lateral-apical to the ventral membranes to form hemidesmosomes. The purpose of this paper is to examine, by using biopsy specimens from BP patients (12 cases), whether or not this internalization of BPAG2 is generated in situ. The fates of BPAG2, 230 kDa BPA (BPAG1) and bound BP-IgG were traced by immunofluorescence microscopy using monoclonal antibodies to BPAG1, BPAG2 and human IgG. In more than half of the lesional and perilesional biopsy specimens, internalization of BPAG2 into the basal cells was observed, while in normal skin BPAG2 was detected on the whole surface of the basal cells without its internalization. No internalization of BPAG1 was detec...Continue Reading

References

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Citations

Apr 24, 1999·The British Journal of Dermatology·S SatohY Kitajima
Jun 7, 2014·Medical Molecular Morphology·Toshiyuki OzawaDaisuke Tsuruta
Apr 18, 2012·The Journal of Dermatology·Daisuke TsurutaTakashi Hashimoto
Jan 23, 2013·The American Journal of Pathology·Sho HiroyasuDaisuke Tsuruta
Oct 25, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hiroaki IwataHiroshi Shimizu
Dec 5, 1998·Microscopy Research and Technique·Y Hirako, K Owaribe
Nov 15, 2018·The Journal of Pathology·Hiroaki IwataHiroshi Shimizu
Apr 19, 2017·Experimental Dermatology·Hiroaki Iwata, Hideyuki Ujiie
Jun 8, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kelly N MessinghamJanet A Fairley
May 9, 2013·Experimental Dermatology·Hiroaki Iwata, Yasuo Kitajima
Sep 28, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hideyuki UjiieHiroshi Shimizu
Nov 17, 2019·Seminars in Immunopathology·Christian D Sadik, Enno Schmidt
Sep 24, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wataru NishieLeena Bruckner-Tuderman
Oct 16, 2020·Journal of the European Academy of Dermatology and Venereology : JEADV·A LambertsB Horváth

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