Internalization of the dengue virus is cell cycle modulated in HepG2, but not Vero cells

Journal of Medical Virology
Waranyoo Phoolcharoen, Duncan R Smith

Abstract

While many studies have investigated the relationship between cell type and dengue virus infection, no study to date has examined the effect of cell physiology on permissiveness to infection. Unsynchronized and artificially synchronized cell populations at different stages of the cell cycle of two cell types (Vero and HepG2) were examined for permissiveness to infection by two dengue virus serotypes (serotypes 2 and 3) by determining both the levels of virus produced as well as the percentage of cells infected. Vero cells showed no significant differences between either viral production or percentage of cells infected as compared to unsynchronized cells for any of the phases investigated, although production of virus (for both serotypes 2 and 3) was somewhat lower for cells infected during S phase. In contrast, HepG2 cells were significantly more permissive for both infection and virus production in the G(2) phase as compared to other phases examined and serotype differences in permissiveness to infection were noted with cells in the M phase of the cell cycle. These results suggest that the cell cycle may be a mediator of cell permissiveness in some cell types.

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Feb 18, 2006·Transactions of the Royal Society of Tropical Medicine and Hygiene·S L SeneviratneH J de Silva
Mar 11, 2009·Journal of Biomedical Science·Lukkana SuksanpaisanDuncan R Smith
Jun 23, 2010·International Journal of Biological Sciences·Mudiana MuhamadRohana Yusof
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Feb 17, 2015·World Journal of Clinical Cases·Jayanta Samanta, Vishal Sharma
Jan 24, 2020·Expert Review of Anti-infective Therapy·Po Ying ChiaYee Sin Leo

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