Internally controlled PCR system for detection of airborne microorganisms

Journal of Environmental Monitoring : JEM
Evgeny V Usachev, Igor E Agranovski

Abstract

Recently, we reported the outcomes of feasibility studies of a technological approach allowing rapid detection of a wide range of bioaerosols by combining a personal bioaerosol sampler with a real-time PCR technology. The protocol was found suitable for detection of targeted microorganisms within relatively short time periods. Considering the crucial importance of the PCR procedure quality control, the current paper reports the results of the development of an internally controlled PCR system for utilization by the above technology. The suggested strategy is based on utilization of only two fluorescent dyes, which are used respectively for target and internal amplification control (IAC) DNA amplification. A bacteriophage T4 and recombinant phage fd (M13) were used in this research as target and IAC, respectively. The constructed IAC was added directly to the collection liquid of the personal bioaerosol sampler enabling quality control to be present throughout the entire sampling-analysis procedures. For performance evaluation, serial ten-fold dilutions of T4 phage were aerosolized and sampled over a 10 minutes time period. The results showed that T4 phage could be reliably detected at the concentration of around 200 PFU per lit...Continue Reading

References

Feb 24, 2001·Analytical Chemistry·P BelgraderM A Northrup
Jun 6, 2003·Biosensors & Bioelectronics·James A HigginsRonald P Koopman
Jan 30, 2004·Letters in Applied Microbiology·J HoorfarP Fach
Dec 3, 2004·Applied and Environmental Microbiology·Burkhard MalornyReiner Helmuth
Jan 18, 2005·Molecular and Cellular Probes·Laurie J HartmanDavid A Norwood
Mar 3, 2005·Biosensors & Bioelectronics·Benjamin J HindsonJohn M Dzenitis
Oct 15, 2005·Clinical Microbiology Reviews·Daniel V LimMarianne F Kramer
Aug 29, 2006·Biosensors & Bioelectronics·Olivier LazckaF Xavier Muñoz
Oct 21, 2009·Clinics in Laboratory Medicine·Frederick L Kiechle, Carol A Holland
Aug 3, 2010·Lab on a Chip·Pavel NeuzilChunyan Zhang
May 24, 2011·Applied and Environmental Microbiology·Akihiko HataHiroaki Furumai
Aug 23, 2011·International Journal of Medical Microbiology : IJMM·Dorothea WiemerRalf Matthias Hagen

❮ Previous
Next ❯

Citations

Jan 6, 2016·International Journal of Environmental Research and Public Health·Maxim SheludchenkoHelen Stratton
Aug 5, 2014·Analytical Chemistry·David KovářPetr Skládal
Jun 4, 2013·Journal of Applied Microbiology·E V UsachevI E Agranovski
Jun 16, 2015·Frontiers in Microbiology·Anurag SharmaSuresh K Mittal
Oct 7, 2016·Journal of Applied Microbiology·I E AgranovskiO V Usacheva
Apr 12, 2015·Journal of Laboratory Automation·Christopher F Fronczek, Jeong-Yeol Yoon
Feb 9, 2016·The Analyst·Qi LiuGuodong Sui
Jul 13, 2021·Journal of Hazardous Materials·Jyoti BhardwajJaesung Jang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.