International multicenter examination of MOG antibody assays.

Neurology. Neuroimmunology and Neuroinflammation
Markus ReindlPatrick Waters

Abstract

To compare the reproducibility of 11 antibody assays for immunoglobulin (Ig) G and IgM myelin oligodendrocyte glycoprotein antibodies (MOG-IgG and MOG-IgM) from 5 international centers. The following samples were analyzed: MOG-IgG clearly positive sera (n = 39), MOG-IgG low positive sera (n = 39), borderline negative sera (n = 13), clearly negative sera (n = 40), and healthy blood donors (n = 30). As technical controls, 18 replicates (9 MOG-IgG positive and 9 negative) were included. All samples and controls were recoded, aliquoted, and distributed to the 5 testing centers, which performed the following antibody assays: 5 live and 1 fixed immunofluorescence cell-based assays (CBA-IF, 5 MOG-IgG, and 1 MOG-IgM), 3 live flow cytometry cell-based assays (CBA-FACS, all MOG-IgG), and 2 ELISAs (both MOG-IgG). We found excellent agreement (96%) between the live CBAs for MOG-IgG for samples previously identified as clearly positive or negative from 4 different national testing centers. The agreement was lower with fixed CBA-IF (90%), and the ELISA showed no concordance with CBAs for detection of human MOG-IgG. All CBAs showed excellent interassay reproducibility. The agreement of MOG-IgG CBAs for borderline negative (77%) and particular...Continue Reading

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Citations

Jun 20, 2020·Neurology·Patrick Waters, Jacqueline Palace
Aug 1, 2020·Expert Opinion on Emerging Drugs·Ankelien DuchowJudith Bellmann-Strobl
Mar 22, 2020·Neurology. Neuroimmunology and Neuroinflammation
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Jul 31, 2020·Cellular & Molecular Immunology·Alicia ZouFabienne Brilot
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Dec 5, 2020·Journal of the Neurological Sciences·Shabeer PaulImtiyaz Ahmad Bhat
Jan 8, 2021·Neurology. Neuroimmunology and Neuroinflammation·Josep DalmauScott S Zamvil
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Jun 17, 2021·Neurology. Neuroimmunology and Neuroinflammation·Kathrin SchandaMarkus Reindl
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Mar 12, 2021·Brain : a Journal of Neurology·Caterina MacriniEdgar Meinl
Aug 22, 2021·Lancet Neurology·Romain MarignierBernhard Hemmer

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Methods Mentioned

BETA
ELISA
fluorescence-activated cell sorting
FACS
ELISAs

Software Mentioned

GraphPad Prism
SPSS

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