Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas

The Plant Cell
Fantao KongYonghua Li-Beisson

Abstract

Plants and algae must tightly coordinate photosynthetic electron transport and metabolic activities given that they often face fluctuating light and nutrient conditions. The exchange of metabolites and signaling molecules between organelles is thought to be central to this regulation but evidence for this is still fragmentary. Here, we show that knocking out the peroxisome-located MALATE DEHYDROGENASE2 (MDH2) of Chlamydomonas reinhardtii results in dramatic alterations not only in peroxisomal fatty acid breakdown but also in chloroplast starch metabolism and photosynthesis. mdh2 mutants accumulated 50% more storage lipid and 2-fold more starch than the wild type during nitrogen deprivation. In parallel, mdh2 showed increased photosystem II yield and photosynthetic CO2 fixation. Metabolite analyses revealed a >60% reduction in malate, together with increased levels of NADPH and H2O2 in mdh2 Similar phenotypes were found upon high light exposure. Furthermore, based on the lack of starch accumulation in a knockout mutant of the H2O2-producing peroxisomal ACYL-COA OXIDASE2 and on the effects of H2O2 supplementation, we propose that peroxisome-derived H2O2 acts as a regulator of chloroplast metabolism. We conclude that peroxisomal M...Continue Reading

Citations

Mar 6, 2019·The Plant Journal : for Cell and Molecular Biology·Ruizi LiYuefeng Guan
Sep 29, 2019·Cells·Adrien BurlacotYonghua Li-Beisson
Sep 2, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jihyeon LeeYoungsook Lee
Oct 6, 2020·Frontiers in Plant Science·Adrien BurlacotGilles Peltier
Oct 6, 2020·Frontiers in Plant Science·Krzysztof Zienkiewicz, Agnieszka Zienkiewicz
Mar 7, 2021·International Journal of Molecular Sciences·Francisco J CorpasJosé M Palma
Feb 1, 2019·Progress in Lipid Research·Yonghua Li-BeissonJohn L Harwood
Feb 16, 2021·Plant Physiology·Luisa M SandalioMaria C Romero-Puertas
May 25, 2021·The New Phytologist·Yonghua Li-BeissonByung-Ho Kang

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