Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells.

Frontiers in Cell and Developmental Biology
Ishita S MehtaJoanna M Bridger

Abstract

This study demonstrates, and confirms, that chromosome territory positioning is altered in primary senescent human dermal fibroblasts (HDFs). The chromosome territory positioning pattern is very similar to that found in HDFs made quiescent either by serum starvation or confluence; but not completely. A few chromosomes are found in different locations. One chromosome in particular stands out, chromosome 10, which is located in an intermediate location in young proliferating HDFs, but is found at the nuclear periphery in quiescent cells and in an opposing location of the nuclear interior in senescent HDFs. We have previously demonstrated that individual chromosome territories can be actively and rapidly relocated, with 15 min, after removal of serum from the culture media. These chromosome relocations require nuclear motor activity through the presence of nuclear myosin 1β (NM1β). We now also demonstrate rapid chromosome movement in HDFs after heat-shock at 42°C. Others have shown that heat shock genes are actively relocated using nuclear motor protein activity via actin or NM1β (Khanna et al., 2014; Pradhan et al., 2020). However, this current study reveals, that in senescent HDFs, chromosomes can no longer be relocated to expec...Continue Reading

References

Jun 1, 1976·Experimental Cell Research·Y Mitsui, E L Schneider
May 31, 1990·Nature·C B HarleyC W Greider
Mar 1, 1987·Mechanisms of Ageing and Development·B M Stanulis-Praeger
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S W SherwoodR T Schimke
Dec 1, 1987·Mechanisms of Ageing and Development·R P SinghalG L Eichhorn
Jun 3, 1983·Science·V L Wilson, P A Jones
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·G P DimriO Pereira-Smith
Sep 1, 1995·Experimental Cell Research·R C AllsoppC B Harley
Mar 24, 1998·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·J M BridgerP Lichter
Jun 9, 1999·Nature·P G ShielsA E Schnieke
Jun 15, 1999·The Journal of Cell Biology·J A CroftW A Bickmore
Aug 5, 1999·Experimental Gerontology·G PawelecA Engel
Feb 19, 2000·Current Biology : CB·J M BridgerW A Bickmore
Jun 27, 2000·Biophysical Journal·H B SunH Yokota
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Apr 11, 2001·Experimental Gerontology·J Campisi
Sep 27, 2001·Cell·E H Blackburn
Nov 28, 2001·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·M CremerT Cremer
May 2, 2003·Nature Reviews. Cancer·Judith Campisi
May 31, 2003·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Archil B ChkhotuaWalter F Grigioni
Jul 12, 2003·Nature Cell Biology·Simona ParrinelloJudith Campisi
Jul 31, 2003·Cell·Kenkichi MasutomiWilliam C Hahn
Dec 1, 1961·Experimental Cell Research·L HAYFLICK, P S MOORHEAD
Nov 11, 2003·Nature·Fabrizio d'Adda di FagagnaStephen P Jackson
Jan 1, 2004·The Journal of Clinical Investigation·Ittai Ben-Porath, Robert A Weinberg
Jan 17, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Masayuki OgamiMakiko Ueda
Jan 28, 2004·Cancer Research·In Hae KwakIn Kyoung Lim
Nov 3, 2004·The Journal of Clinical Investigation·Janakiraman KrishnamurthyNorman E Sharpless
Aug 17, 2006·Aging Cell·Sigrun ResslerMeinhard Wlaschek
Dec 1, 2006·Nature·Raffaella Di MiccoFabrizio d'Adda di Fagagna
Jan 26, 2007·Nature·Karen J Meaburn, Tom Misteli
Feb 6, 2007·Aging Cell·Karen J MeaburnJoanna M Bridger

❮ Previous
Next ❯

Software Mentioned

erosion
IPLab
Imaris
Quips Pathvysion Capture VP
Digital
TOPHAT
SEQMONK
ENSEMBL

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.