PMID: 11920602Mar 29, 2002Paper

Interphase FISH assays for the detection of translocations with breakpoints in immunoglobulin light chain loci

International Journal of Cancer. Journal International Du Cancer
Jose I Martín-SuberoReiner Siebert

Abstract

Many B-cell malignancies bear chromosomal translocations juxtaposing immunoglobulin (IG) genes with oncogenes, resulting in deregulated expression of the latter. Translocations affecting the IG heavy chain (IGH) locus in chromosomal region 14q32 are most prevalent. However, variant translocations involving the IG kappa (IGK) locus in 2p12 or the IG lambda (IGL) locus in 22q11 occur recurrently in B-cell neoplasias. No routine methods for the detection of all breakpoints involving IG light chain loci independently of the translocation partner have been described. For this reason, we have designed 2 novel interphase fluorescence in situ hybridization (FISH) assays using differentially labeled probes flanking the IGK and IGL locus, respectively. Based on extensive control studies, the diagnostic thresholds for the detection of breakpoints were set at 0.3% for IGK and 1.4% for IGL. Fifteen cases of B-cell malignancies with cytogenetically detectable chromosomal abnormalities in 2p11-14 were investigated with the FISH assay for IGK. Breakpoints affecting the IGK locus were detected in 7 cases including all 4 variant Burkitt's translocations t(2;8)(p12;q24) and a variant BCL2-associated translocation t(2;18)(p12;q21). Other transloca...Continue Reading

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Citations

Aug 7, 2002·The American Journal of Pathology·José Ignacio Martín-SuberoReiner Siebert
Dec 13, 2005·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Xavier SagaertChristiane De Wolf-Peeters
Jun 9, 2006·The New England Journal of Medicine·Michael HummelUNKNOWN Molecular Mechanisms in Malignant Lymphomas Network Project of the Deutsche Krebshilfe
Apr 12, 2011·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Christel Eckmann-ScholzAlmuth Caliebe
Oct 1, 2015·Leukemia & Lymphoma·Bo-Jung ChenShih-Sung Chuang
Jun 9, 2007·Cancer Genetics and Cytogenetics·Renata WoronieckaPrzemyslaw Janik
Jun 29, 2005·Histopathology·J F GarcíaM A Piris
Nov 20, 2008·British Journal of Haematology·Inga VaterReiner Siebert
Nov 2, 2012·European Journal of Haematology·Masaya ShimanukiHideki Nakakuma
Jan 4, 2012·International Journal of Cancer. Journal International Du Cancer·René ScholtysikRalf Küppers
Feb 6, 2004·Pathology·Dominic V SpagnoloJenny Turner
Aug 1, 2006·The American Journal of Pathology·Christoph RennéAndreas Bräuninger
Dec 24, 2005·Pathology·Alberto Catalano, Harry Iland

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