Interplay between Alternative Splicing and Alternative Polyadenylation Defines the Expression Outcome of the Plant Unique OXIDATIVE TOLERANT-6 Gene

Scientific Reports
Qingshun Q LiMan Liu

Abstract

Pre-mRNA alternative splicing and alternative polyadenylation have been implicated to play important roles during eukaryotic gene expression. However, much remains unknown regarding the regulatory mechanisms and the interactions of these two processes in plants. Here we focus on an Arabidopsis gene OXT6 (Oxidative Tolerant-6) that has been demonstrated to encode two proteins through alternative splicing and alternative polyadenylation. Specifically, alternative polyadenylation at Intron-2 of OXT6 produces a transcript coding for AtCPSF30, an Arabidopsis ortholog of 30 kDa subunit of the Cleavage and Polyadenylation Specificity Factor. On the other hand, alternative splicing of Intron-2 generates a longer transcript encoding a protein named AtC30Y, a polypeptide including most part of AtCPSF30 and a YT521B domain. To investigate the expression outcome of OXT6 in plants, a set of mutations were constructed to alter the splicing and polyadenylation patterns of OXT6. Analysis of transgenic plants bearing these mutations by quantitative RT-PCR revealed a competition relationship between these two processes. Moreover, when both splice sites and poly(A) signals were mutated, polyadenylation became the preferred mode of OXT6 processing...Continue Reading

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Citations

Jun 7, 2018·Molecular Biology of the Cell·Ha Neul LeeHilary A Coller
Feb 3, 2021·Journal of Experimental & Clinical Cancer Research : CR·Yi ZhangPengyuan Liu
Oct 25, 2019·Genes & Diseases·Fuwen YuanQianben Wang
Jan 21, 2019·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Soizik BerlivetCécile Bousquet-Antonelli

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Methods Mentioned

BETA
transgenic
Rapid Amplification
PCR
electrophoresis

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