Interplay between grain structure and protein adsorption on functional response of osteoblasts: ultrafine-grained versus coarse-grained substrates

Journal of Biomedical Materials Research. Part a
R D K MisraL P Karjalainen

Abstract

The rapid adsorption of proteins is the starting and primary biological response that occurs when a biomedical device is implanted in the physiological system. The biological response, however, depends on the surface characteristics of the device. Considering the significant interest in nano-/ultrafine surfaces and nanostructured coatings, we describe here, the interplay between grain structure and protein adsorption (bovine serum albumin: BSA) on osteoblasts functions by comparing nanograined/ultrafine-grained (NG/UFG) and coarse-grained (CG: grain size in the micrometer range) substrates by investigating cell-substrate interactions. The protein adsorption on NG/UFG surface was beneficial in favorably modulating biological functions including cell attachment, proliferation, and viability, whereas the effect was less pronounced on protein adsorbed CG surface. Additionally, immunofluorescence studies demonstrated stronger vinculin signals associated with actin stress fibers in the outer regions of the cells and cellular extensions on protein adsorbed NG/UFG surface. The functional response followed the sequence: NG/UFG(BSA) > NG/UFG > CG(BSA) > CG. The differences in the cellular response on bare and protein adsorbed NG/UFG and ...Continue Reading

References

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S A Santoro, L W Cunningham
Oct 1, 1979·Cell Biology International Reports·F GrinnellW Snell
Jun 1, 1978·Cell·L B ChenM L Walsh
Jan 1, 1992·Biomaterials·J J Collis, G Embery
Aug 1, 1991·Biomaterials·J E Ellingsen
Jun 1, 1987·Journal of Biomedical Materials Research·P B van WachemW G van Aken
May 1, 1987·The Journal of Prosthetic Dentistry·T Albrektsson, M Jacobsson
May 1, 1988·Biomaterials·R L Williams, D F Williams
Nov 1, 1983·The Journal of Cell Biology·A S CurtisF Lawrie
Mar 1, 1981·The Journal of Cell Biology·H K KleinmanG R Martin
Dec 1, 1981·Thrombosis Research·F Grinnell, M K Feld
Jan 1, 1981·Collagen and Related Research·E RuoslahtiE G Hayman
Dec 1, 1980·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·G PottA Sieber
Nov 1, 1980·Mechanisms of Ageing and Development·J LindenaF Deerberg
Mar 1, 1981·The Journal of Investigative Dermatology·F GrinnellL Burgess
May 1, 1981·Journal of Biomedical Materials Research·F Grinnell, M K Feld
Mar 1, 1981·Journal of Biomedical Materials Research·R LangerD Tapper
Jul 11, 1980·JAMA : the Journal of the American Medical Association·K M Yamada
Dec 1, 1995·Calcified Tissue International·J NordahlF P Reinholt
Dec 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R G WinnardR T Franceschi
Aug 17, 1999·Journal of Biomedical Materials Research·G DaculsiG Guicheux
Jul 6, 2000·Journal of Biomedical Materials Research·T J WebsterR Bizios
Oct 1, 2003·Journal of Biomedical Materials Research. Part a·Yunzhi YangJoo L Ong
Oct 20, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·S R SousaM A Barbosa
Jun 1, 2005·Journal of Biomedical Materials Research. Part a·G ZhaoB D Boyan
Aug 16, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Veronica BorsariRoberto Giardino
Dec 20, 2005·Journal of Materials Science. Materials in Medicine·S R SousaM A Barbosa
Jan 18, 2011·Colloids and Surfaces. B, Biointerfaces·A Dolatshahi-PirouzF Besenbacher
Mar 5, 2011·Colloids and Surfaces. B, Biointerfaces·Hanna M KowalczyńskaRobert Kołos

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Citations

Dec 18, 2015·Scientific Reports·Yu YanLijie Qiao
Jun 19, 2013·Journal of Biomedical Materials Research. Part a·C NuneL P Karjalainen
Jul 1, 2014·Journal of Orthopaedic Translation·Jun MaDonghui Zhu
Mar 5, 2015·Journal of Materials Science. Materials in Medicine·Xiao-Lin CaoYonghao Yu
Sep 28, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Chang YeVijay K Vasudevan
Mar 30, 2017·Biomedical Materials·Young Jung NoHala Zreiqat
Apr 24, 2014·ACS Applied Materials & Interfaces·Sara BagherifardMario Guagliano

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