Interplay between histone H3 lysine 56 deacetylation and chromatin modifiers in response to DNA damage

Genetics
Antoine SimoneauHugo Wurtele

Abstract

In Saccharomyces cerevisiae, histone H3 lysine 56 acetylation (H3K56Ac) is present in newly synthesized histones deposited throughout the genome during DNA replication. The sirtuins Hst3 and Hst4 deacetylate H3K56 after S phase, and virtually all histone H3 molecules are K56 acetylated throughout the cell cycle in hst3∆ hst4∆ mutants. Failure to deacetylate H3K56 causes thermosensitivity, spontaneous DNA damage, and sensitivity to replicative stress via molecular mechanisms that remain unclear. Here we demonstrate that unlike wild-type cells, hst3∆ hst4∆ cells are unable to complete genome duplication and accumulate persistent foci containing the homologous recombination protein Rad52 after exposure to genotoxic drugs during S phase. In response to replicative stress, cells lacking Hst3 and Hst4 also displayed intense foci containing the Rfa1 subunit of the single-stranded DNA binding protein complex RPA, as well as persistent activation of DNA damage-induced kinases. To investigate the basis of these phenotypes, we identified histone point mutations that modulate the temperature and genotoxic drug sensitivity of hst3∆ hst4∆ cells. We found that reducing the levels of histone H4 lysine 16 acetylation or H3 lysine 79 methylation...Continue Reading

References

Jun 25, 1976·Journal of Molecular Biology·V JacksonR Chalkley
Oct 10, 1975·Science·A Ruiz-CarrilloV G Allfrey
Dec 1, 1992·Molecular and Cellular Biology·N KouprinaF Spencer
Dec 22, 1999·The Journal of Cell Biology·A TaddeiG Almouzni
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·J LandryR Sternglanz
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·J S SmithJ D Boeke
Jun 22, 2000·Yeast·V V Kushnirov
Jan 5, 2001·Nature·J A DownsS P Jackson
Nov 21, 2001·Nature Cell Biology·A A AlcasabasS J Elledge
Jun 28, 2002·Cell·Fred van LeeuwenDaniel E Gottschling
Aug 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Christine M SmithDaniel E Gottschling
Sep 24, 2002·Nature Genetics·Jeong-Hyun ParkJef D Boeke
Nov 14, 2002·Cell Cycle·Joanna Bloom, Michele Pagano
Mar 11, 2003·The Journal of Biological Chemistry·Ann SuttonRolf Sternglanz
Jun 7, 2003·Science·Lee Zou, Stephen J Elledge
Jul 29, 2004·DNA Repair·Edgardo R Parrilla-CastellarLarry Karnitz
Sep 24, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Xiang-Jiao Yang
Dec 1, 2004·Annual Review of Genetics·Berit Olsen Krogh, Lorraine S Symington
Jan 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Adolfo SaiardiSolomon H Snyder
Apr 13, 2005·The Journal of Cell Biology·Alan J TackettBrian T Chait
May 13, 2005·The Journal of Biological Chemistry·Anil OzdemirColin Logie
Aug 9, 2005·Current Biology : CB·Frédéric D SweeneyDaniel Durocher
Sep 17, 2005·Molecular and Cellular Biology·Robert WysockiStephen J Kron
Sep 20, 2005·Current Biology : CB·Achille Pellicioli, Marco Foiani
Feb 9, 2006·The Journal of Biological Chemistry·Laura J BensonAnthony T Annunziato
Feb 21, 2006·Current Opinion in Cell Biology·Hugo Wurtele, Alain Verreault

❮ Previous
Next ❯

Citations

Oct 18, 2016·Cell Cycle·Bingbing WanXiaolan Zhao
Jul 12, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Lina WangWei Li
Aug 21, 2020·Molecular Reproduction and Development·Yu-Guo YuanChen Qian
Aug 17, 2020·Epigenetics : Official Journal of the DNA Methylation Society·Anagh RayRonita Nag Chaudhuri
Jan 31, 2020·Frontiers in Cell and Developmental Biology·Zsolt KarányiLóránt Székvölgyi
Apr 28, 2021·Epigenetics : Official Journal of the DNA Methylation Society·Lei FangChunyuan Jin
Jun 5, 2021·DNA Repair·Ian Hammond-MartelHugo Wurtele
Sep 25, 2021·Applied Microbiology and Biotechnology·Tatsuya KatoEnoch Y Park

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.