Interplay between intrinsically disordered proteins inside membraneless protein liquid droplets.

Chemical Science
Yongsang Jo, Yongwon Jung

Abstract

Intrinsically disordered proteins (IDPs) in cells phase separate to form diverse membraneless organelles, which have condensed liquid droplet-like properties and often contain multiple IDPs. However, how potential interactions between different IDPs affect the dynamic behavior of these protein droplets is largely unknown. Here, we develop a rapid IDP clustering system to generate protein droplets with varied residue compositions and examine diverse interacting IDPs inside droplets. Three different IDP droplets actively recruited other diverse IDPs inside droplets with extremely varied enrichment (inside/outside) degrees (over 100-fold variation) under highly crowded conditions. The recruited IDPs were mostly mobile even inside highly immobile droplets. Among the five tested IDPs, the disordered region of Ddx4 helicase with its unique multiple charged residue blocks was noticeably influenced by droplet mobility. We also discovered that droplets of different IDPs could rapidly fuse to each other. Interestingly, some droplets were heterogeneously fused with segregated subcompartments, and this segregation was enhanced by droplet maturation and was more apparent for specific IDP pairs, in which the polar and charged residue composi...Continue Reading

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Citations

Jun 24, 2020·Emerging Topics in Life Sciences·M Sankaranarayanan, Timothy T Weil
Jan 30, 2021·Physical Review Letters·Andrew T R ChristyMark A Miller
Jan 31, 2021·Biophysical Journal·Jonas AhlersMartina Havenith
Aug 6, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Denisa Jamecna, Bruno Antonny
Jan 20, 2021·ACS Biomaterials Science & Engineering·Cristobal Garcia GarciaKristi L Kiick

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Methods Mentioned

BETA
fluorescence recovery after photobleaching
Fluorescence
fluorescence imaging

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