Interplay Between Lipid Modulators of Kir2 Channels: Cholesterol and PIP2

Computational and Structural Biotechnology Journal
Avia Rosenhouse-DantskerIrena Levitan

Abstract

We have shown earlier that Kir2 channels are suppressed by the elevation of membrane cholesterol. Moreover, it is also well known that activation of Kir channels is critically dependent on a regulatory phospholipid, phosphatidylinositol-4,5-bisphosphate (PIP2). In this study we examined the cross-talk between cholesterol and PIP2 in the regulation of Kir2 channels. The strength of Kir2-PIP2 interactions was assessed by acute sequestering of PIP2 with neomycin dialyzed into cells through a patch pipette while simultaneously recording whole cell currents. Consistent with a reduction in PIP2 levels, dialysis of neomycin resulted in a decrease in Kir2.1 and Kir2.3 current amplitudes (current rundown), however, this effect was significantly delayed by cholesterol depletion for both types of channels suggesting that cholesterol depletion strengthens the interaction between Kir2 channels and PIP2. Furthermore, mutation of Kir2.1 that renders the channels' cholesterol insensitive abrogated cholesterol depletion-induced delay in the current rundown whereas reverse mutation in Kir2.3 has the opposite effect. These observations provide further support for the functional cross-talk between cholesterol and PIP2 in regulating Kir2 channels. ...Continue Reading

References

Dec 9, 1985·Biochimica Et Biophysica Acta·P L Yeagle
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Dec 14, 1999·The Journal of Biological Chemistry·T RohácsD E Logothetis
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Stuart McLaughlinDiana Murray
Jun 28, 2002·Neuron·Coeli M B LopesDiomedes E Logothetis
Nov 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jeanne KwikMichael Edidin
Oct 7, 2004·Biophysical Journal·Victor G RomanenkoIrena Levitan
Sep 8, 2005·The Journal of Biological Chemistry·Decha EnkvetchakulColin G Nichols
May 15, 2007·The Journal of Physiology·Diomedes E LogothetisAvia Rosenhouse-Dantsker
May 24, 2007·Pflügers Archiv : European journal of physiology·Diomedes E LogothetisAvia Rosenhouse-Dantsker
Sep 11, 2009·The Journal of Biological Chemistry·Dev K SinghIrena Levitan
Nov 20, 2009·Channels·Avia Rosenhouse-DantskerIrena Levitan
Jan 21, 2010·Physiological Reviews·Hiroshi HibinoYoshihisa Kurachi
Apr 17, 2010·Pflügers Archiv : European journal of physiology·Diomedes E LogothetisRahul Mahajan
Oct 6, 2010·The Journal of Biological Chemistry·Nazzareno D'AvanzoColin G Nichols
Jan 20, 2011·Biophysical Journal·Avia Rosenhouse-DantskerIrena Levitan
Jul 30, 2011·Biochimica Et Biophysica Acta·Dev K SinghIrena Levitan
Nov 20, 2012·Frontiers in Physiology·Zhongkui HongGabor Forgacs
Sep 11, 2013·The Journal of Biological Chemistry·Avia Rosenhouse-DantskerIrena Levitan
Mar 13, 2014·Frontiers in Physiology·Irena LevitanAvia Rosenhouse-Dantsker
Jul 20, 2014·The Journal of Physiology·Huazhi HanIrena Levitan

❮ Previous
Next ❯

Citations

Apr 6, 2017·Biochimica Et Biophysica Acta. Biomembranes·Anna N Bukiya, Avia Rosenhouse-Dantsker
May 3, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Maria SanchoDonald G Welsh
Mar 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Anna L DuncanMark S P Sansom
Dec 27, 2017·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·José J De Jesús-PérezJorge Arreola
Apr 15, 2017·Biochimica Et Biophysica Acta. Biomembranes·José A PovedaJosé M González-Ros
Apr 5, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Florina ZakanyZoltan Varga

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection

Software Mentioned

Pulse

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Yulia EpshteinIrena Levitan
The Journal of Biological Chemistry
Dev K SinghIrena Levitan
© 2022 Meta ULC. All rights reserved