Abstract
The ribosomal protein L11 (RPL11) integrates different types of stress into a p53-mediated response. Here, we analyzed the impact of the ubiquitin-like protein SUMO on the RPL11-mouse double-minute 2 homolog-p53 signaling. We show that small ubiquitin-related modifier (SUMO)1 and SUMO2 covalently modify RPL11. We find that SUMO negatively modulates the conjugation of the ubiquitin-like protein neural precursor cell-expressed developmentally downregulated 8 (NEDD8) to RPL11 and promotes the translocation of the RP outside of the nucleoli. Moreover, the SUMO-conjugating enzyme, Ubc9, is required for RPL11-mediated activation of p53. SUMOylation of RPL11 is triggered by ribosomal stress, as well as by alternate reading frame protein upregulation. Collectively, our data identify SUMO protein conjugation to RPL11 as a new regulator of the p53-mediated cellular response to different types of stress and reveal a previously unknown SUMO-NEDD8 interplay.-El Motiam, A., Vidal, S., de la Cruz-Herrera, C. F., Da Silva-Álvarez, S., Baz-Martínez, M., Seoane, R., Vidal, A., Rodríguez, M. S., Xirodimas, D. P., Carvalho, A. S., Beck, H. C., Matthiesen, R., Collado, M., Rivas, C. Interplay between SUMOylation and NEDDylation regulates RPL11 loca...Continue Reading
References
Apr 29, 1993·Nature·J D OlinerB Vogelstein
May 15, 1997·Nature·Y HauptM Oren
May 15, 1997·Nature·M H KubbutatK H Vousden
Feb 5, 1998·FEBS Letters·R HondaH Yasuda
Sep 12, 1998·Molecular Cell·J M DesterroR T Hay
Nov 24, 1999·The EMBO Journal·M S RodriguezR T Hay
Nov 24, 1999·The EMBO Journal·M GostissaG Del Sal
Feb 29, 2000·The Journal of Biological Chemistry·H Saitoh, J Hinchey
Jul 13, 2000·Cell·T BuschmannZ Ronai
Sep 26, 2002·FEBS Letters·Dimitris P XirodimasRonald T Hay
Jul 5, 2003·Cancer Cell·Marion A E LohrumKaren H Vousden
Aug 15, 2003·Oncogene·Lihong Chen, Jiandong Chen
Nov 13, 2003·Molecular and Cellular Biology·Yanping ZhangYue Xiong
Jan 31, 2004·Nucleic Acids Research·Yan LingKeith D Robertson
May 21, 2004·The EMBO Journal·Krishna P BhatYanping Zhang
May 29, 2004·The Journal of Biological Chemistry·Weidong ZhouHuilin Zhou
Aug 17, 2004·The Journal of Biological Chemistry·Mu-Shui Dai, Hua Lu
May 7, 2005·Cell Cycle·Nadia BarbouleAnnie Valette
May 7, 2005·Cell Cycle·Helen RizosRichard F Kefford
Jun 29, 2006·The Journal of Biological Chemistry·Mu-Shui DaiHua Lu
Sep 27, 2006·Molecular & Cellular Proteomics : MCP·Alfred C O VertegaalAngus I Lamond
Nov 2, 2006·The Journal of Biological Chemistry·Marek SramkoJuraj Bies
May 20, 2009·Cell·Jan-Philipp Kruse, Wei Gu
Jun 26, 2009·Journal of Virology·Laura Marcos-VillarCarmen Rivas
Aug 29, 2009·EMBO Reports·Anders SundqvistDimitris P Xirodimas
May 14, 2010·The Biochemical Journal·Kevin A Wilkinson, Jeremy M Henley
Jun 26, 2010·Cell Death and Differentiation·M CampagnaC Rivas
Apr 3, 2012·The Journal of Biological Chemistry·Mu-Shui DaiHua Lu
Sep 28, 2012·Cell Death & Disease·J González-SantamaríaC Rivas
Oct 15, 2013·Cell Reports·Katherine E SloanNicholas J Watkins
Jan 29, 2014·IUBMB Life·Chun Guo, Jeremy M Henley
Apr 8, 2014·Oncogene·C F de la Cruz-HerreraC Rivas
Feb 19, 2015·Journal of Molecular Cell Biology·Shan WangLingqiang Zhang
Mar 4, 2015·Cell Reports·Kazuho NishimuraKeiji Kimura
Apr 14, 2015·Oncogene·A BaillyD P Xirodimas
Jun 24, 2015·Cell Division·Jorrit M Enserink
Nov 10, 2015·Cell Reports·Patricia DominguesBenjamin G Hale
Jan 24, 2017·Nature Structural & Molecular Biology·Ivo A HendriksMichael L Nielsen
Citations
Oct 9, 2019·Cells·Sergey O Sulima, Jonathan D Dinman
Feb 2, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Frédéric LessardGerardo Ferbeyre
Oct 11, 2020·Wiley Interdisciplinary Reviews. RNA·Cyrielle PetibonSherif Abou Elela
Jan 21, 2021·Cell Reports·Sofia Lobato-GilDimitris P Xirodimas
Apr 26, 2020·Seminars in Cell & Developmental Biology·Na LiCong-Yi Wang
Dec 24, 2020·Trends in Cancer·Jessie S Kroonen, Alfred C O Vertegaal