Interplay of lncRNA H19/miR-675 and lncRNA NEAT1/miR-204 in breast cancer

Molecular Oncology
Volkmar MüllerHeidi Schwarzenbach

Abstract

Long noncoding RNAs (lncRNAs) are frequently precursor RNAs of microRNAs (miRNAs) or act as competing endogenous RNAs (ceRNAs) to interact with miRNAs. To better understand the shared impact of lncRNAs and miRNAs in the regulatory post-transcriptional network, we focused here on the relationships between (a) lncRNA H19 and miR-675, (b) NEAT1 and miR-204, and (c) HOTAIR and miR-331 in plasma of early breast cancer (BC) patients. We quantified each RNA in plasma samples of 63 BC patients and 10 healthy women by quantitative real-time PCR. In cell culture experiments, the influence of these noncoding RNAs (ncRNAs) on proliferation and apoptosis of BC cell line MCF-7 was examined. Plasma levels of H19 (P = 0.030), NEAT1 (P = 0.030), and miR-331 (P = 0.012) were deregulated in BC patients compared with healthy women. In both cohorts, the concentrations of H19 correlated with those of miR-675 (P = 0.0001). Higher H19 (P = 0.001) along with lower miR-675 (P = 0.007) levels and higher miR-204 (P = 0.017) along with lower NEAT1 (P = 0.030) levels were detected in plasma of HER2-positive patients compared with the other BC subgroups. Whereas the expression of HOTAIR was below the detection level, miR-331 levels correlated with nodal stat...Continue Reading

References

Jan 1, 1990·Molecular and Cellular Biology·C I BrannanS M Tilghman
Jun 30, 2005·The Journal of Biological Chemistry·Nathalie BerteauxEric Adriaenssens
Jan 24, 2007·RNA·Xuezhong Cai, Bryan R Cullen
Feb 29, 2008·Genome Biology·Julia Höck, Gunter Meister
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Jun 6, 2012·Nature Structural & Molecular Biology·Marc R Fabian, Nahum Sonenberg
Jun 27, 2013·International Journal of Cancer. Journal International Du Cancer·Sung Mi KimTae Yong Jeon
Feb 5, 2014·Nature Reviews. Clinical Oncology·Heidi SchwarzenbachKlaus Pantel
May 16, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rui-Min ChangLian-Yue Yang
Jun 27, 2014·Seminars in Cell & Developmental Biology·Je-Hyun YoonMyriam Gorospe
Sep 23, 2014·Advanced Drug Delivery Reviews·Paloma Del C MonroigGeorge A Calin
Oct 16, 2014·RNA·Laura StandaertJean-Christophe Marine
Nov 12, 2014·Acta Biochimica Et Biophysica Sinica·Xiaolong ZhouWenru Tang
Jul 24, 2015·Expert Review of Molecular Diagnostics·Heidi Schwarzenbach
Sep 27, 2015·Clinical Chemistry·Heidi SchwarzenbachKlaus Pantel
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yaoyong LuRongwei Liao
Apr 14, 2016·Cancer Cell·Adam M Schmitt, Howard Y Chang
May 6, 2016·Gene Regulation and Systems Biology·Hao KeBaowei Jiao
Aug 20, 2016·Cancer Biomarkers : Section a of Disease Markers·Kaijiong ZhangJinbo Liu
Nov 15, 2016·EBioMedicine·Heidi Schwarzenbach
Jul 20, 2017·International Journal of Biological Macromolecules·Xuerui LiLinzhu Wen
Sep 28, 2017·Cancer Biomarkers : Section a of Disease Markers·Dahua ZhaoNing Yu
Aug 22, 2018·Molecular Therapy. Nucleic Acids·Asghar ArshiMohammad Mahdi Ghahramani Seno
Dec 19, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Dongzhi HuDalu Kong

❮ Previous
Next ❯

Citations

Jan 31, 2020·Frontiers in Oncology·Esraa A SalamaHend M El Tayebi
Mar 20, 2020·Molecular Cancer·Francesca CrudeleStefano Volinia
Apr 4, 2021·International Journal of Molecular Sciences·Dana DvorskáZuzana Danková
May 27, 2021·Cancer Cell International·Yitong LiuChengkun Wang
Aug 11, 2021·Cancer Cell International·Qian LuHongming Pan
Sep 21, 2021·Molecular Biotechnology·Yongxiang LiuJinglong Yan
Oct 17, 2021·Journal of Hematology & Oncology·Yiyin ZhangHui Lin

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
PCR
Assay
transfection
biopsy
FACS
xenograft

Software Mentioned

SPSS
FlexiTube
AllStars

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis