Interplay of microtubule dynamics and sliding during bipolar spindle formation in mammalian cells.

Current Biology : CB
Swapna KolluDuane A Compton

Abstract

Accurate chromosome segregation during mitosis relies on the organization of microtubules into a bipolar spindle. Kinesin-5 proteins play an evolutionarily conserved role in establishing spindle bipolarity [1, 2] and clinical trials are currently evaluating inhibitors of human kinesin-5 (i.e., Eg5) for chemotherapeutic potential. However, in mammalian somatic cells, Eg5 activity is dispensable for maintenance of bipolar spindles once they are formed [3, 4], suggesting distinct requirements for establishment versus maintenance of spindle bipolarity. By combining Eg5 inhibition with RNA interference of other spindle proteins, we show that mitotic cells deficient in MCAK fail to maintain spindle bipolarity in the absence of Eg5 activity. Collapse of bipolar spindles in MCAK-deficient cells is driven by pole-focusing activities and is independent of MCAK function at centromeres, implicating hyperstabilized non-kinetochore microtubules in spindle collapse. Conversely, destabilizing nonkinetochore microtubules in early mitosis reduces the reliance on Eg5 for establishment of spindle bipolarity and renders cells partially resistant to Eg5 inhibitors. Thus, the temporal requirement for microtubule sliding generated by Eg5 activity duri...Continue Reading

References

Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M A JordanL Wilson
Dec 1, 1993·The Journal of Cell Biology·D N MastronardeJ R McIntosh
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·L BerruetaB E Bierer
Apr 20, 2000·The Journal of Biological Chemistry·C SpittleL Cassimeris
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·G J Mack, D A Compton
Jun 26, 2002·The Journal of Cell Biology·Cristiana MollinariRobert L Margolis
Aug 16, 2002·Molecular Biology of the Cell·Susan L Kline-Smith, Claire E Walczak
Nov 12, 2003·The Journal of Cell Biology·Eileen T O'TooleThomas Müller-Reichert
Jun 21, 2007·Current Biology : CB·Adam M SaundersWilliam M Saxton
Aug 1, 2007·The Journal of Cell Biology·Kerstin H TheinUlrike Gruneberg
Oct 16, 2007·Nature Cell Biology·Ge YangTarun M Kapoor
Nov 28, 2007·The Journal of Cell Biology·Linda WordemanGeorge von Dassow
Mar 26, 2008·Biochemical and Biophysical Research Communications·Jian DuGregory J Hannon
Dec 9, 2008·Nature Cell Biology·Samuel F BakhoumDuane A Compton
Jan 23, 2009·Molecular Biology of the Cell·Rania S RizkClaire E Walczak
Feb 11, 2009·The Journal of Cell Biology·Alberto TosoAndrew D McAinsh
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Benjamin R HoughtalingTarun M Kapoor

❮ Previous
Next ❯

Citations

Apr 28, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Vidya C NadarPeter W Baas
May 9, 2014·Molecular Biology of the Cell·A Sophia Gayek, Ryoma Ohi
Oct 10, 2012·The Journal of Cell Biology·Ana R R MaiaHelder Maiato
Aug 21, 2013·Gene·Edward J WojcikSunyoung Kim
Sep 7, 2014·Biochemical and Biophysical Research Communications·Fang Wang, Stanley Li Lin
Aug 26, 2011·Bioarchitecture·Marvin E TanenbaumAnna Akhmanova
Feb 22, 2012·Current Biology : CB·Greg FitzHarris
Nov 19, 2013·Current Biology : CB·Viktoriya SyrovatkinaPhong T Tran
Dec 15, 2010·Developmental Cell·Marvin E Tanenbaum, René H Medema
Jun 28, 2016·Biochimica Et Biophysica Acta·Kozo Tanaka, Toru Hirota
Jun 30, 2016·Chromosoma·Roy G H P van HeesbeenRené H Medema
Mar 28, 2013·Environmental and Molecular Mutagenesis·Mario FioreFrancesca Degrassi
Dec 16, 2021·Journal of Cell Science·Aaron R Tipton, Gary J Gorbsky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.