Interpolated twitches in fatiguing single mouse muscle fibres: implications for the assessment of central fatigue.

The Journal of Physiology
Nicolas PlaceHåkan Westerblad

Abstract

An electrically evoked twitch during a maximal voluntary contraction (twitch interpolation) is frequently used to assess central fatigue. In this study we used intact single muscle fibres to determine if intramuscular mechanisms could affect the force increase with the twitch interpolation technique. Intact single fibres from flexor digitorum brevis of NMRI mice were dissected and mounted in a chamber equipped with a force transducer. Free myoplasmic [Ca2+] ([Ca2+](i)) was measured with the fluorescent Ca2+ indicator indo-1. Seven fibres were fatigued with repeated 70 Hz tetani until 40% initial force with an interpolated pulse evoked every fifth tetanus. Results showed that the force generated by the interpolated twitch increased throughout fatigue, being 9 +/- 1% of tetanic force at the start and 19 +/- 1% at the end (P < 0.001). This was not due to a larger increase in [Ca2+](i) induced by the interpolated twitch during fatigue but rather to the fact that the force-[Ca2+](i) relationship is sigmoidal and fibres entered a steeper part of the relationship during fatigue. In another set of experiments, we observed that repeated tetani evoked at 150 Hz resulted in more rapid fatigue development than at 70 Hz and there was a decr...Continue Reading

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Citations

Apr 27, 2010·European Journal of Applied Physiology·Nicolas PlaceHåkan Westerblad
Jul 8, 2010·European Journal of Applied Physiology·Maria PapaiordanidouAlain Varray
Aug 26, 2011·European Journal of Applied Physiology·Nicola A MaffiulettiRoberto Bottinelli
Aug 2, 2008·Clinical and Experimental Pharmacology & Physiology·Nicolas PlaceHåkan Westerblad
May 8, 2013·PloS One·Olivier GirardSébastien Racinais
Aug 31, 2010·Journal of Electromyography and Kinesiology : Official Journal of the International Society of Electrophysiological Kinesiology·Pierre-David PetitSerge S Colson
Feb 14, 2009·Journal of Electromyography and Kinesiology : Official Journal of the International Society of Electrophysiological Kinesiology·Nicolas PlaceAlain Martin
Oct 12, 2010·Muscle & Nerve·Nicolas PlaceNicola A Maffiuletti
Mar 30, 2016·The Journal of General Physiology·Thomas Holm PedersenOle Bækgaard Nielsen
Mar 8, 2011·Annals of Physical and Rehabilitation Medicine·S Boyas, A Guével
Jan 26, 2017·Sports Medicine·Gabriel S TrajanoAnthony J Blazevich
Jul 2, 2009·Journal of Applied Physiology·Astrid M Horstman
Jan 5, 2012·Medicine and Science in Sports and Exercise·Daria NeyroudNicolas Place
Nov 13, 2019·Sports Medicine·Nicolas Place, Guillaume Y Millet
Mar 1, 2014·Biophysics Reviews·Juan C CalderónCarlo Caputo
Aug 12, 2009·Journal of Applied Physiology·Simon C Gandevia
May 10, 2021·European Journal of Applied Physiology·Raffy DotanPaola Contessa
May 16, 2021·European Journal of Applied Physiology·Nicolas Place

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